Journal: Journal of Pharmacy Research

Article Id: JPRS-P'Col-00002057
Title: Anticancer activity of the tropical fern, Pyrrosia heterophylla (L.) M.G. Price on human breast cancer cell line through p53-p21 mediated cell cycle arrest and downregulation of anti-apoptotic gene
Category: Pharmacology
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    Aim: Pyrrosia heterophylla (L.) M.G. Price is a tropical epiphytic fern used in Indian traditional and tribal medicine for the treatment of various diseases. The present study evaluated the apoptotic potential of hexane (PHH), ethyl acetate (PHE), and methanolic (PHM) extracts of the plant. Methods: Cytotoxicity screening of the extracts was done using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and acridine orange-ethidium bromide staining on three human cancer cell lines (MCF7, HT-29, and HeLa). Based on this, PHE was selected for further studies on the mechanism of apoptotic induction in MCF7 cells. Ability to induce DNA fragmentation was assessed by comet assay. Flow cytometric analysis helped to quantify the apoptotic cells and to study the effects of PHE on cell cycle. Expression analysis of pro- and anti- apoptotic genes was done to elucidate the mechanism of action of the selected extract. Gas chromatography-mass spectrometry analysis of the selected extract was done to identify the phytocompounds present in it. Results: PHE was found to be the most effective extract with an IC50 of 49.99 ± 0.61 µg/ml on MCF7 cells. At IC50 concentration, PHE could effectively induce apoptosis in about 51.24% of the treated cells. It was capable of inducing DNA fragmentation in MCF7 cells as revealed by the characteristic “hedgehog” tails in the comet assay. PHE significantly upregulated p53 and p21 which resulted in the suspension of the cell cycle at G0/G1 phase. Downregulation of anti-apoptotic genes Bcl-2 and Bcl-xl by p53 also helped in inducing apoptosis. Many bioactive phytochemicals including eugenol and phytol were identified in PHE. Conclusion: The study could identify the anticancer potential of P. heterophylla and elucidate the mechanisms of its apoptotic activity.

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    Author(s) Name:

    M. George1 *, L. Joseph1 , V. S. Josekumar1,2

    Affiliation(s) Name:

    1Department of Zoology, Mar Ivanios College (Autonomous), Thiruvananthapuram, Kerala, India,

    2 Department of Biology, Arba Minch University, Ethiopia

    *Corresponding author: Meera George, Department of Zoology, Mar Ivanios College (Autonomous), Thiruvananthapuram-- 695015, Kerala, India.

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    Author:

    M. George1 *, L. Joseph1 , V. S. Josekumar1,2

    Title:Anticancer activity of the tropical fern, Pyrrosia heterophylla (L.) M.G. Price on human breast cancer cell line through p53-p21 mediated cell cycle arrest and downregulation of anti-apoptotic gene
    Journal:Journal of Pharmacy Research
    Vol(issue):12 (April)
    Year:2018
    Page No: (526-535)
  • Experimental Methods Keywords

    Methodology:Anticancer activity, Apoptotic, Bcl-2, Breast cancer, p21, p53
    Research Materials:Pyrrosia heterophylla

Keywords

Apoptotic Bcl-2 Breast cancer p21 p53 Pyrrosia heterophylla

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