Journal: Drug Invention Today

Article Id: JPRS-BRPT-00002073
Title: Immunogenic activity of chicken antibodies toward Anopheles dirus salivary gland antigens
Category: Biotechnology & Related Pharmaceutical Technology
Section: Research Article
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    Introduction: Immunogenic salivary proteins of Anopheles dirus were foremost studied for their allergenic properties. Most of the saliva antigens were found to be species specific as only weak cross-reactivity was predicted to depend on different host system for their survival. Aim: In this study, we have examined the applicability for IgY obtained from the screening of different kinds of hen eggs, for its capability to interact towards a mosquito antigenic protein. Methods: Due to the peculiar composition of the egg yolk, IgY are normally purified using complex and time-consuming procedures involving a combination of precipitation and chromatographic steps. Soluble proteins were separated from the lipidic fraction of egg yolk by various methods and loaded onto PEG column. Results: High recovery and purity of IgY was obtained for normal and brown eggs by water dilution and chloroform methods. Similarly, IgY is also purified in extend of 90% with chromatography and was tested against salivary gland antigens after various treatments. The active antibodies were found to be 178.57ug/ml for brown egg and 200ug/ml for normal egg towards the antigen. Conclusion: Although the difference in both egg source for the IgY is significant, normal egg antibodies has shown highest purity and interaction towards the antigen.

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    Author(s) Name:

    Mounika Sajja*, Sudhakar Johnson Tangirala, Kavya Krishna Veerapaneni

    Affiliation(s) Name:

    Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India

    *Corresponding author: Mounika Sajja, Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh - 522 502, India.

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    Author:

    Mounika Sajja*, Sudhakar Johnson Tangirala, Kavya Krishna Veerapaneni

    Title:Immunogenic activity of chicken antibodies toward Anopheles dirus salivary gland antigens
    Journal:Drug Invention Today
    Vol(issue):10 (Jan)
    Year:2018
    Page No: (85-88)
  • Experimental Methods Keywords

    Methodology:Immunogenic activity, Chromatography, IgY, Quantitative precipitin assay, Radioimmunoassay
    Research Materials:Anopheles dirus

Keywords

Anopheles dirus Chromatography IgY Quantitative precipitin assay Radioimmunoassay

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