Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-00002088
Title: Chancroid vaccine: Solving the puzzle within a proteome of Haemophilus ducreyi to reach the target vaccine leads by reverse vaccinology approach
Category: Pharmaceutical Biotechnology
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    Aim: Haemophilus ducreyi - a causative agent of chancroid disease in human - has been studied for vaccine lead search by reverse vaccinology approach to record the cell surface antigens and their epitopes for the high scored values as per conserved nature and ability to span plasma membrane and cell wall. Materials and Methods: With the combined use of the TMHMM, LipoP1.0, SignalP4.1, PSORTB, BLASTP, HLA Pred, and T-cell epitope designer servers, most conserved and promising epitopes available in the proteome of the H. ducreyi have been recorded. Result: The study highlighted that of 1717 protein encoded by the H. ducreyi, only 12 proteins available as cell surface proteins found to be qualifying as per the protocol and we reported their epitopes as best scored peptides possibly been the most useful in vaccine designing and in vitro studies. Conclusion: Using the reverse vaccinology approach, 12 most conserved epitopes and their binding energy with HLA molecule have been reported for the H. ducreyi and considered as vaccine leads for future studies.

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    Author(s) Name:

    Shreya Fadnavis1 , Dilip Gore*2 , Maithili Hedaoo1 , Mayur Dange1 , M. A. Soni 1 , A. P. Kopulwar1

    Affiliation(s) Name:

    1 Department of Biotechnology, Priyadarshini Institute of Engineering and Technology, Mouza Shivangaon, Behind CRPF Campus, Hingna Road, Nagpur – 440 019, Maharashtra, India,

    2 Department of Bioinformatics, Sai Biosystems Private Limited, Plot No. 271 Raghuji Nagar, Nagpur – 440 009, Maharashtra, India

    *Corresponding Author: Dilip Gore, Department of Bioinformatics, Sai Biosystems Private Limited, Plot No. 271 Raghuji Nagar, Nagpur – 440 019, Maharashtra, India

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    Author:

    Shreya Fadnavis1 , Dilip Gore*2 , Maithili Hedaoo1 , Mayur Dange1 , M. A. Soni 1 , A. P. Kopulwar1

    Title:Chancroid vaccine: Solving the puzzle within a proteome of Haemophilus ducreyi to reach the target vaccine leads by reverse vaccinology approach
    Journal:Journal of Pharmacy Research
    Vol(issue):12(4)
    Year:2018
    Page No: (636-640)
  • Experimental Methods Keywords

    Methodology:vaccine, reverse vaccinology, epitope, conserved nature
    Research Materials: Haemophilus ducreyi

Keywords

Haemophilus ducreyi vaccine reverse vaccinology epitope conserved nature

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