Journal: Drug Invention Today

Article Id: JPRS-PDA-00002099
Title: Method development and validation for the assay of dipyridamole extended-release capsules by reverse-phase high-performance liquid chromatography method
Category: Pharmaceutical Drug Analysis
Section: Research Article
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    Aim: The aim of the current study is to develop and validate a novel analytical method for the assay of dipyridamole (DPM) extended-release capsules by reverse-phase high-performance liquid chromatography (RP-HPLC) method. Materials and Methods: The current study describes an optimized assay method and forced degradation studies (acid, alkaline, thermal, peroxide, and water) for this drug. A RP-HPLC has been developed in an optimized way. The chromatographic separation was achieved on Agilent Eclipse plus C18 (100 × 4.6 mm × 5 µm) column with phosphate buffer (pH 7.0) and methanol:0.01N HCl (50:50% v/v) which was used as a mobile phase with an effluent flow rate of 1.2 ml/min. The detection was carried out at 295 nm. Results and Discussion: The retention time was found to be 8.59 min. This method has been validated for linearity, accuracy, and precision. Linearity was found to be in the concentration range of 0.2–0.8 mg/ml. The drug percentage recovery was found to be in the range of 99.34–100.92% of the test concentration. The results of the limit of detection and limit of quantification were within the acceptance limits. Conclusion: DPM is widely used to prevent strokes and vascular thrombosis. The developed RP-HPLC method was found to be accurate, precise, selective, and sensitive for the analysis of DPM extended-release capsules 200 mg.

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    Author(s) Name:

    Kella Alekhya*, Afroz Patan, M. Vijey Aanandhi

    Affiliation(s) Name:

    Department of Pharmaceutical Chemistry and Analysis, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamil Nadu, India

    *Corresponding author: Kella Alekhya, Department of Pharmaceutical Chemistry and Analysis, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 603 117, Tamil Nadu, India.

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    Author:

    Kella Alekhya*, Afroz Patan, M. Vijey Aanandhi

    Title:Method development and validation for the assay of dipyridamole extended-release capsules by reverse-phase high-performance liquid chromatography method
    Journal:Drug Invention Today
    Vol(issue):10 (Feb)
    Year:2018
    Page No: (157-164)
  • Experimental Methods Keywords

    Methodology:Method validation, Reverse-phase high-performance liquid chromatography, Vasodilator agent
    Research Materials: Dipyridamole

Keywords

Dipyridamole Extended-release capsule dosage form Method validation Reverse-phase high-performance liquid chromatography Vasodilator agent

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