Journal: Journal of Pharmacy Research

Article Id: JPRS-PP-0000210
Title: Optimization of SFE extraction of three sesquiterpenes from Syringa pinnatifolia Hemsl using orthogonal test design and subsequent isolation by HSCCC chromatography
Category: Pharmacognosy and Phytochemistry,
Section: Research Article
  • Abstract
  • Audio Abstract
  • Authors
  • Pdf File
  • Citation
  • My Reference
  • Methodology
  • Abstract

    Objective: In the present study, we investigated the Optimization of SFE extraction of three sesquiterpenes from Syringa pinnatifolia Hemsl using orthogonal test design and subsequent isolation by HSCCC chromatography. Methods: An orthogonal L9(3)4 test design was applied to select the optimum extraction parameters including pressure, temperature, modifier and sample particle size on yield using an analytical-scale SFE system. The crude extract from preparative SFE was successfully isolated and purified by preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of n-hexane–chloroform–methanol–water (10:11:11:10, v/v) by increasing the flow-rate of the mobile phase stepwise from 1.2 to 2.2 ml/min after 120 min. Results: The process was scaled up by 80 times using a preparative SFE system under the optimized conditions of 20 Mpa of pressure, 30 °C of temperature, 40-60 mesh of sample particle size and modified CO2 with 20% methanol. The target compounds isolated and purified by HSCCC were analyzed by high-performance liquid chromatography (HPLC). The separation produced total of 50.8 mg of 15-norcadina-2-en-5,9-diol at 99.0% purity, 35.4 mg of guai-9-en-4â-ol at 98.5% purity and 39.0 mg of 14,15-dinorguai-1,11-dien-9,10-dione at 98.8% purity from 200 mg of the crude extract in one-step separation. Conclusion: The sesquiterpenes were isolated from Syringa pinnatifolia Hemsl may serve as a potential source of useful drugs in the near future.

  • Abstract Audio

    Click Here

  • About the authors and Affiliations

    Author(s) Name:

    Qinghu Wang* , Nayintai Dai, Narenchaoketu Han

    Affiliation(s) Name:

    College of Traditional Mongolian Medicine, Inner Mongolia University for Nationalities, Tongliao 028000, Inner Mongolia, China

    *Corresponding author. 

    Qinghu Wang 

    College of Traditional Mongolian Medicine,

    Inner Mongolia University for Nationalities,

    Tongliao 028000, Inner Mongolia, China


    Received on:07-01-2014 ; Accepted on:31-05-2014

  • View Article File in pdf format.

    Article File
  • View Article Citation Here.

    0 View More
  • How to Cite my Article.

    Author:

    Qinghu Wang* , Nayintai Dai, Narenchaoketu Han

    Title:Optimization of SFE extraction of three sesquiterpenes from Syringa pinnatifolia Hemsl using orthogonal test design and subsequent isolation by HSCCC chromatography
    Journal:Journal of Pharmacy Research
    Vol(issue):8 (June)
    Year:2014
    Page No: (707-713)
  • Experimental Methods Keywords

    Methodology:SFE, HSCCC,HPLC
    Research Materials:Syringa pinnatifolia Hemsl

Keywords

Syringa pinnatifolia Hemsl Sesquiterpene SFE HSCCC HPLC

Our Services

Most Downloaded List