Journal: Drug Invention Today

Article Id: JPRS-DA-00002102
Title: Validation of Cefpodoxime proxetil and Ambroxol hydrochloride by reverse-phase high-performance liquid chromatographic method
Category: Drug Analysis
Section: Research Article
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    Objective: A simple, accurate, precise reverse-phase high-performance liquid chromatographic method was developed for simultaneous estimation of Cefpodoxime proxetil and hydrochloride HCl in tablet dosage form. Methods: Chromatogram was run through Phenomenex C18 (250 × 4.6 mm, 5 µm) with a mobile phase consisting acetonitrile:methanol:water (30:30:40 v/v/v) at a flow rate 1 ml/min, 0.025M potassium dihydrogen phosphate as buffer, and pH 4.0 with orthophosphoric acid. UV-spectrometry detection was measured at 245 nm. The developed method was validated in terms of accuracy, linearity, precision, limit of detection (LOD), limit of quantification, and solution stability. Results: The retention time of Cefpodoxime and Ambroxol HCl was found to be 2.35 min and 4.37 min and % RSD of Cefpodoxime proxetil and Ambroxol HCl was found to be 1.02% and 0.28%, respectively. The described method shows good linearity range of 30–150 μg/ml Cefpodoxime proxetil and 20–100 μg/ml for Ambroxol HCl. The correlation coefficient of Cefpodoxime proxetil and Ambroxol HCl was 0.999 and 0.999. The LOD was found to be 2.97 ppm and 3.04 ppm and the limit of quantitation values were 10.1 ppm and 10 ppm, respectively. % assay was obtained as 99.6% and 101.4 % for Cefpodoxime proxetil and Ambroxol HCl. Conclusion: The results of the study showed that the proposed reverse-phase high-performance chromatographic method was simple, rapid, precise, and accurate which is useful in the routine determination of Cefpodoxime proxetil and Ambroxol HCl bulk drug and its pharmaceutical dosage form.

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    Author(s) Name:

    Sunkara Namratha1 , A. Vijayalakshmi 2 *

    Affiliation(s) Name:

    1 Department of Analysis, Bharat Group of Institutions, Ibrahimpatnam, Hyderabad, Telangana, India,

    2 Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamil Nadu, India

    *Corresponding author: A. Vijayalakshmi, Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Pallavaram, Chennai - 600 117, Tamil Nadu, India.

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    Author:

    Sunkara Namratha1 , A. Vijayalakshmi 2 *

    Title:Validation of Cefpodoxime proxetil and Ambroxol hydrochloride by reverse-phase high-performance liquid chromatographic method
    Journal:Drug Invention Today
    Vol(issue):10 (Feb)
    Year:2018
    Page No: (174-178)
  • Experimental Methods Keywords

    Methodology: Estimation, Limit of detection, Limit of quantitation, Simultaneous
    Research Materials:Ambroxol HCl, Cefpodoxime proxetil,

Keywords

Ambroxol HCl Cefpodoxime proxetil Estimation Limit of detection Limit of quantitation Simultaneous

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