Journal: Journal of Pharmacy Research

Article Id: JPRS-PSPA-00002146
Title: Phytochemical screening and in vitro antioxidants activities of ethanolic extract of Acokanthera schimperi leaves
Category: Phytochemical Study and Pharmacological Activity
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    Objective: Acokanthera schimperi belonging to the family Apocynaceae is well known in Ethiopia to treat skin diseases, tonsillitis, and snake bites for its antimicrobial, antiseptic, and anti-inflammatory properties, and it has a long history of being used as an herbal remedy. The present study was aimed to analyze the phytochemical screening and in vitro antioxidant activities of the organic leaf extracts, to find out novel antioxidants in a pharmaceutical formulation to treat oxidative stress- related diseases without side effects. Methods: Leaves of A. schimperi were subjected to successive solvent extraction using petroleum ether, chloroform, ethyl acetate, and ethanol, to obtain the respective extracts, these extracts were used for phytochemical screening using standard qualitative phytochemical methods, then ethanolic extracts were tested for antioxidant activity using various in vitro antioxidant methods such as 1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), hydroxyl (OH), superoxide radical (SOR) scavenging, and reducing power activity. Results: Qualitative phytochemical analysis of leaves of A. schimperi organic extracts revealed that the maximum presence of flavonoids, terpenoids, phenolic compounds, tannins, saponins, glycosides, and phytosterol. Further in vitro, antioxidant activity of ethanolic extract from leaves of A. schimperi, among various antioxidant assays performed the maximum inhibition observed for DPPH for 80% with IC50 of 53±12μg/ml, followed by NO for 73% with IC50 53.48 μg/ml, superoxide radical for 60% with IC50 of 91.08μg/ml. Conclusion: The results of the present study demonstrated that ethanolic leaf extract of A. schimperi possess significant bioactive secondary metabolites with potential antioxidant activity, so these leaves of the plant used as a viable source of natural antioxidants for industrial and pharmaceutical preparations.

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    Author(s) Name:

    Berhe Abrha1 , Krishna Chaithanya K1 *, Gopalakrishnan V K1 , Zenebe Hagos1 , Mulugeta Hiruy1 , Devaki K2

    Affiliation(s) Name:

    1 Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia,

    2 Department of Biochemistry, Karpagam University, Coimbatore, India

    *Corresponding author: Dr. Krishna Chaithanya Karri, Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Post Box. No 1010, Axum, Tigray Region, Ethiopia.

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    Author:

    Berhe Abrha1 , Krishna Chaithanya K1 *, Gopalakrishnan V K1 , Zenebe Hagos1 , Mulugeta Hiruy1 , Devaki K2

    Title:Phytochemical screening and in vitro antioxidants activities of ethanolic extract of Acokanthera schimperi leaves
    Journal:Journal of Pharmacy Research
    Vol(issue):12(5)
    Year:2018
    Page No: (660-666)
  • Experimental Methods Keywords

    Methodology:Free radical, Oxidative stress, Antioxidant activity
    Research Materials:Acokanthera schimperi

Keywords

Free radical Oxidative stress Antioxidant Acokanthera schimperi

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