Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-00002147
Title: Inherent immunological activity of chicken antibodies toward Anopheles punctipennis salivary gland antigens as early biomarkers for low-level infestation of malaria
Category: Pharmaceutical Biotechnology
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    Introduction: Malaria is a major public health concern in most of the Asian and Sub-Saharan Africa, including Kenya, with Anopheles punctipennis, Anopheles dirus, Anopheles gambiae, Anopheles arabiensis and Anopheles funestus as the principal vectors. Flocks that receive high mosquito exposure will be most effective for surveillance purposes. However, mosquito population indices at the flock sites may only provide an indirect measure of potential exposure. Aim: In this study, we have examined the applicability for IgY obtained from the screening of different kinds of hen eggs, for its capability to interact towards a mosquito antigenic protein. Methods: The growing diffusion of monoclonal and polyclonal antibodies in immunotherapy and immuno-diagnostics and the strict standards for animal care have led to a renewed interest in the use of chickens for antibody production, as opposed to mammals. Egg yolk represents an economical source of polyclonal antibodies since the concentration of IgY is similar or higher than in the serum of chickens or rabbits or humans reaching levels ranging from 15 to 25 mg IgY per ml of yolk in the case of hyperimmunized hen. Results: Soluble proteins were separated from the lipidic fraction of egg yolk by various methods and loaded onto polyethylene glycol-column. High recovery and purity of IgY were obtained for Guinea fowl eggs by charcoal and sodium alginate methods and for Polish hen eggs by charcoal method. Conclusion: Although the difference in egg sources for the IgY is significant and shown extreme interaction towards the antigen, development of antimalarial agents represents technical challenges and immense opportunities for improvement of global health.

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    Author(s) Name:

    Maheswara Reddy Mallu1 *, Kavya Krishna Veerapaneni 1 , Shrinivasulu Kamma1 , Nithin Chand Kurra2

    Affiliation(s) Name:

    1 Centre for Bioprocess Engineering, Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India,

    2 Siddhartha Medical College, Dr. NTR University of Health Sciences, Vijayawada, Andhra Pradesh, India

    *Corresponding author: Maheswara Reddy Mall, Centre for Bioprocess Technology, Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Guntur, Andhra Pradesh, India

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    Author:

    Maheswara Reddy Mallu1 *, Kavya Krishna Veerapaneni 1 , Shrinivasulu Kamma1 , Nithin Chand Kurra2

    Title:Inherent immunological activity of chicken antibodies toward Anopheles punctipennis salivary gland antigens as early biomarkers for low-level infestation of malaria
    Journal:Journal of Pharmacy Research
    Vol(issue):12 (May)
    Year:2018
    Page No: (667-672)
  • Experimental Methods Keywords

    Methodology:Antigen, Enzyme-linked immunosorbent assay, Guinea fowl, IgY, Lipoprotein
    Research Materials: Anopheles punctipennis

Keywords

Anopheles Antigen Enzyme-linked immunosorbent assay Guinea fowl IgY Lipoprotein

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