Journal: Drug Invention Today

Article Id: JPRS-BMa-00002183
Title: Anti-inflammatory Activity of Bioactive Flavonoid Apigenin- 7-O-β-D- Glucuronide Methyl Ester from Ethyl Acetate Leaf Extract of Manilkara zapota on Lipopolysaccharide- induced Pro-inflammatory Medi
Category: Biological Macromolecules
Section: Research Article
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    Introduction: Apigenin-7-O-β-D-glucuronide (AG) is a bioactive flavonoid isolated from the leaves of ethyl extract of Manilkara zapota, it is well known to exhibit significant in vitro and in vivo anti-inflammatory activity. Objective: The present study was aimed to evaluate the in vitro anti-inflammatory activity of AG against nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS) induced RAW 264.7 macrophages. Materials and Methods: Measurement of pro-inflammatory mediators such as NO and PGE2 in the RAW 264.7 macrophages using enzyme-linked immunosorbent assay. Results: AG, exhibited dose-dependent inhibitory effect on the production of pro-inflammatory mediators such as NO and PGE2 in LPS-stimulated RAW 264.7 macrophages. Conclusion: From this study, it can be concluded that the isolated bioactive AG, act as potent anti-inflammatory agents, it can be used for the treatment of inflammatory related diseases without having any side effects.

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    Author(s) Name:

    Kamalakararao K1 , Gopalakrishnan VK2 , Zenebe Hagos2 , Govinda Rao D3 , Padal SB4 , Krishna Chaithanya K2 *

    Affiliation(s) Name:

    1 Departments of Biochemistry, Adikavi Nannaya University, Rajahmundry, Andhra Pradesh, India,

    2 Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia,

    3 Department of Biochemistry and Molecular Biology, School of Biological Sciences, Central University of Kerala, Kasaragod, Kerala, India 4 Departments of Botany, Andhra University, Visakhapatnam, Andhra Pradesh, India

    *Corresponding author: Dr. Krishna Chaithanya Karri, Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Tigray Region, Post Box No. 1010, Ethiopia.

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    Author:

    Kamalakararao K1 , Gopalakrishnan VK2 , Zenebe Hagos2 , Govinda Rao D3 , Padal SB4 , Krishna Chaithanya K2 *

    Title:Anti-inflammatory Activity of Bioactive Flavonoid Apigenin- 7-O-β-D- Glucuronide Methyl Ester from Ethyl Acetate Leaf Extract of Manilkara zapota on Lipopolysaccharide- induced Pro-inflammatory Medi
    Journal:Drug Invention Today
    Vol(issue):10 (April)
    Year:2018
    Page No: (531-535)
  • Experimental Methods Keywords

    Methodology:Anti-inflammatory agents, Lipopolysaccharide, Macrophage, Nitric oxide, Prostaglandin-E2
    Research Materials:Manilkara zapota

Keywords

Anti-inflammatory agents Lipopolysaccharide Macrophage Nitric oxide Prostaglandin-E2

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