Journal: Drug Invention Today

Article Id: JPRS-D(DD)-00002252
Title: High-performance thin-layer chromatography fingerprinting analysis of bioactive compounds in hydro-alcoholic extracts of polyherbal formulation
Category: Drug (Discovery/Development)
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    Objective: The objective of the study was to explore the fingerprinting profile of bioactive compounds present in hydro- alcoholic extract of polyherbal formulation (HAF). Materials and Methods: High-performance thin layer chromatography (HPTLC) fingerprinting of HAF was performed and calculate Rf values at wavelength 580 nm. HAF consists of a hydro- alcoholic extract of 11 medicinal plants which have protective activity against cellular damage related activity. HPTLC method was performed to evaluate the presence of polyphenolics employing mobile phase n-hexane:ethyl acetate:formic acid (4:5:1, v/v/v). Results: Peaks were symmetrical in nature and no tailing was observed when plates were scanned at wavelength 580 nm. The Rf values of HAF at sample volume 25 µl (70 mg in 7 ml methanol + HCl) seen maximum in nos. with corresponding to percentage of peak area were observed to be 0.035 (1.23%), 0.113 (8.25%), 0.189 (7.57%), 0.254 (3.72%), 0.291 (5.37%), 0.381 (10.52%), 0.437 (11.18%), 0.547 (4.49%), 0.597 (5.99%), 0.64 (9.83%), 0.69 (27.35%), and 0.881 (3.22%). HPTLC was performed to confirm the quantitative presence of polyphenolics in extracts of HAF. Conclusions: The present standardization provides a specific and accurate tool to develop a confirmatory fingerprinting profile (HPTLC) of biomarkers present in HAFs for many curative activities.

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    Author(s) Name:

    A. K. Srivastava1 *, D. Kaushik2 , V. K. Lal 3

    Affiliation(s) Name:

    1 Department of Pharmacognosy, Anand College of Pharmacy, Keetham, Agra – 282 007, Uttar Pradesh, India,

    2 Department of Pharmaceutical Chemistry, Agra Public Pharmacy College, Agra - 282 007, Uttar Pradesh, India,

    3 Department of Pharmacognosy, Ex-Deputy Director, CCRAS, Ministry of AYUSH, New Delhi, India.

    *Corresponding author: Anuj Kumar Srivastava, Department of Pharmacognosy, Anand College of Pharmacy, Keetham, Agra – 282 007, Uttar Pradesh, India.

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    Author:

    A. K. Srivastava1 *, D. Kaushik2 , V. K. Lal 3

    Title:High-performance thin-layer chromatography fingerprinting analysis of bioactive compounds in hydro-alcoholic extracts of polyherbal formulation
    Journal:Drug Invention Today
    Vol(issue):10(5)
    Year:2018
    Page No: (805-810)
  • Experimental Methods Keywords

    Methodology:High-performance thin-layer chromatography fingerprinting analysis
    Research Materials:Hydro- alcoholic extract of polyherbal formulation (HAF)

Keywords

Biomarker Peak Polyherbal formulation Polyphenolics Quantification

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