Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002312
Title: Vascular activity of protopanaxadiol and protopanaxatriol, the major saponin fractions isolated from roots of Panax notoginseng
Category: Pharmacology
Section: Research Article
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    Background: Panax notoginseng (PN) (Burk) F. H. Chen (Araliacea) is a Chinese herb commonly known in Asia for its medicinal potentials, antihypertensive included. The aim of this study was to investigate the vasorelaxant activity of protopanaxadiol (PDS) and protopanaxatriol (PTS), the major saponin fractions isolated from roots of PN, and identify the underlying mechanisms on isolated rat aortic rings. Methods: Radnoti Tissue-Organ bath instrument (ADInstruments) was used to perform Tissue-Organ bath experiments, and tensions produced on isolated rat aortic rings were recorded by the help of ADInstruments PowerLab 8/30 system (Model ML 870, Australia). Results: Both PDS and PTS (10−6 −10−3 µg / ml) induced concentration-dependent relaxations in intact rat aortic rings pre-contracted with norepinephrine (1 µM).This activity was considerably reduced after removal of the endothelium and in intact rings pre-treated with NG-nitro-L-arginine methyl ester (L-NAME, 100 μM), 1H-[1,2,4]-oxadiazole-[4,3-α]-quinoxaline-1-one (ODQ, 10 μM), and combination of L-NAME and ODQ. However, pre-treatment with indomethacin (10 μM) did not affect either PDS- or PTS-induced relaxations. Furthermore, both PDS and PTS (10−4 and 10−3 µg /ml) decreased CaCl 2 - and potassium chloride (KCL)-induced vasoconstrictions in a dose-dependent manner. Our findings suggest that both PDS and PTS produced vasorelaxant effect that was mediated by NO-cyclic guanosine monophosphate in intact rings and by blockade of calcium influx in vascular smooth muscle. Cyclooxygenase pathway, on the other hand, had no apparent role. Among these fractions, PDS showed better effect with a slight difference. Conclusion: Findings from this study showed that both PDS and PTS fractions possess vasorelaxant activity. This supports the potential use of PN in management of cardiovascular diseases, especially hypertension.

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    Author(s) Name:

    Rosette Uzayisenga1,2 *, Yi Wang1 , Yu Ren1 , Peter Amwoga Ayeka1,3 , Pramod Singh Kunwar 2 , Epaphrodite Twahirwa2

    Affiliation(s) Name:

    1International Education College, College of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Nankai District, Tianjin, P.R. China,

    2 School of Pharmacy, Mount Kenya University, Thika, Kenya,

    3 Faculty of Science, Egerton University, Egerton, Kenya

    *Corresponding author: Rosette Uzayisenga, School of Pharmacy, Mount Kenya University, P.O. Box 342-0100, Thika, Kenya.

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    Author:

    Rosette Uzayisenga1,2 *, Yi Wang1 , Yu Ren1 , Peter Amwoga Ayeka1,3 , Pramod Singh Kunwar 2 , Epaphrodite Twahirwa2

    Title:Vascular activity of protopanaxadiol and protopanaxatriol, the major saponin fractions isolated from roots of Panax notoginseng
    Journal:Drug Invention Today
    Vol(issue):10 (June)
    Year:2018
    Page No: (1035-1040)
  • Experimental Methods Keywords

    Methodology:Vascular activity
    Research Materials:Panax notoginseng, Protopanaxadiol, Protopanaxatriol

Keywords

Cardiovascular disease Panax notoginseng Protopanaxadiol Protopanaxatriol Vascular relaxation

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