Journal: Drug Invention Today
Title: In vitro antioxidant activities of isolated bioactive flavonoid apigenin-7-O-β-D-glucuronide methyl ester from ethyl acetate leaf extract of Manilkara zapota
Category: Pharmacological Screening
Section: Research Article
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Abstract
Introduction: Apigenin-7-O-β-D-glucuronide methyl ester is a bioactive flavonoid, has been isolated from the ethyl acetate leaf extract of Manilkara zapota, and shown both in vitro and in vivo anti-inflammatory activities. Objective: The present study was aimed to evaluate the in vitro antioxidant activities of bioactive flavonoid apigenin-7-O-β-D-glucuronide methyl ester from the ethyl acetate leaf extract by in vitro antioxidant assays such as 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) assays. Materials and Methods: The in vitro antioxidant assays such as DPPH and NO were carried out using standard methods. Results: The results showed that the apigenin-7-O-β-D-glucuronide methyl ester (25, 50, and 100 μg/mL) has significant DPPH and NO free radical inhibiting activity at 100 µg/ml was 83.26%, 86.19% with half maximal inhibitory concentration (IC50 ) values of 36.38 and 29.74, whereas the standards, ascorbic acid, and 3,5-di-tert- butyl-4-hydroxytoluene at 10 µg/ml, exhibited 54.21% and 56.75% with IC50 values of standards 4.49 µg/ml and 3.93 µg/ml, respectively. Conclusion: From this study, it can be concluded that the apigenin-7-O-β-D-glucuronide methyl ester, exhibited significant scavenging activity on DPPH and NO free radical inhibiting activity, and could serve as a potential source of natural antioxidants, for the development of therapeutic antioxidant drugs.
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About the authors and Affiliations
Author(s) Name: K. Kamalakararao1 , V. K. Gopalakrishnan2 , Zenebe Hagos2 , Y. Satyaprasad3 , Krishna Chaithanya Karri 2 *
Affiliation(s) Name: 1 Department of Biochemistry, Adikavi Nannaya University, Rajahmundry, Andhra Pradesh, India,
2 Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Aksum, Ethiopia,
3 Departments of Chemical Engineering, College of Engineering, Aksum University, Aksum, Ethiopia
*Corresponding author: Dr. Krishna Chaithanya Karri, Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Tigray Region, Post Box No 1010, Ethiopia.
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Author: K. Kamalakararao1 , V. K. Gopalakrishnan2 , Zenebe Hagos2 , Y. Satyaprasad3 , Krishna Chaithanya Karri 2 *
Title: In vitro antioxidant activities of isolated bioactive flavonoid apigenin-7-O-β-D-glucuronide methyl ester from ethyl acetate leaf extract of Manilkara zapota Journal: Drug Invention Today Vol(issue): 10 (July) Year: 2018 Page No: (1142-1146) -
Experimental Methods Keywords
Methodology: 1, 1-diphenyl-2-picrylhydrazyl, Antioxidants, Apigenin-7-O-β-D-glucuronide methyl ester, Manilkara zapota, Nitric oxide Research Materials: Manilkara zapota
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