Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002494
Title: Assessment of urease activity in Pisum sativum seeds
Category: Pharmacology
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    Introduction: Urease is an enzyme which catalyzes urea into ammonia and carbon dioxide. Urease is abundantly found in seeds of Leguminosae family. It is an important enzyme for plants as it converts atmospheric nitrogen into absorbable form of nitrogen. It is even found in some bacteria and fungi and sometimes acts as defense mechanism. This research aims at extracting urease from Pisum sativum seeds. Materials and Methods: The dry seed sample was bought from a local departmental store. The extraction of the enzyme was done in three steps. They are precipitation, dialysis, and electrophoresis. The activity of the protein extracted was checked under changing pH and temperature. The molecular weight of the enzyme was found using sodium dodecyl sulfate-polyacrylamide gel electrophoresis technique. Results: The optimum pH was found to be 7.5, and the optimum temperature was found to be 40°C. The molecular weight of the enzyme was found to be 90kD. Conclusion: Urease was extracted from P. sativum. The molecular weight was estimated. The enzyme activity was checked in changing pH and temperature. Urease can be used to regulate the level of urea in diabetic patients.

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    Author(s) Name:

    Prabhav Kumar Iyer1 , V. Vishnu Priya2 *, R. Gayathri 2

    Affiliation(s) Name:

    1 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India,

    2 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

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    Author:

    Prabhav Kumar Iyer1 , V. Vishnu Priya2 *, R. Gayathri 2

    Title:Assessment of urease activity in Pisum sativum seeds
    Journal:Drug Invention Today
    Vol(issue):10(9)
    Year:2018
    Page No: (1810-1813)
  • Experimental Methods Keywords

    Methodology:Urease activity
    Research Materials:Pisum sativum

Keywords

Urease Pisum sativum Optimum pH Optimum temperature

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