Journal: JPR:BioMedRx:An International Journal

Article Id: JPRS-DVD-0000254
Title: Development and validation of a thin-layer chromatography-densitometric method for the quantitation of Isovanillin from Hemidesmus indicus (Linn.) R.Br., its extracts and marketed formulations
Category: Development and Validation of Drugs
Section: Research Article
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    A selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been proposed for the analysis of Hemidesmus indicus and its formulations for isovanillin content. Hemidesmus indicus is a twining shrub and a root of this taxon has been used in folk medicine as well as in ayurvedic and unani preparations. The method employed TLC aluminium plates precoated with silica gel 60 F254 as the stationary phase. Linear ascending development with toluene: ethyl acetate: methanol: acetic acid 7.5: 1.5: 0.5:0.5 (v/v) as the mobile phase was performed at room temperature (25 ± 2ºC) in a twin-trough glass chamber saturated with mobile phase vapour. Compact bands (Rf 0.39 ± 0.02) were obtained for isovanillin in Crude plant materials, Herbal extracts and Formulations. Scanning was performed in absorbance mode at 230nm. Linear regression analysis of the calibration plots showed good linear relationship between peak area and peak height (r2 = 0.9907 ±0.0002) in the concentration range 87-167 ppm. The method was validated for precision, recovery, robustness, specificity, detection and quantification limits. Recovery percentage in the sample found in between 84.14-86.73%. Quantification values of isovanillin in raw materials, extracts and marketed formulations were found to be 0.17%, 0.40% & 0.14% respectively. The developed technique is precise, specific and accurate for checking the purity of H. indicus.

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    Author(s) Name:

    Rajesh Kumar Verma*, Anubhuti Pasrija, Alok Sharma and Rahul Singh

    Affiliation(s) Name:

    Dabur Research and Development Centre- Analytical Department,Dabur India Ltd. Company, Sahibabad, Ghaziabad, Uttar Pradesh, India

    *Corresponding author.
    Rajesh Kumar Verma
    Drug Research and Development
    Centre- Analytical Department
    Dabur India Ltd. Company,
    Sahibabad, Ghaziabad,
    Uttar Pradesh, India


    Received on:17-10-2012; Revised on: 19-11-2012; Accepted on:10-01-2013

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    Author:

    Rajesh Kumar Verma*, Anubhuti Pasrija, Alok Sharma and Rahul Singh

    Title:Development and validation of a thin-layer chromatography-densitometric method for the quantitation of Isovanillin from Hemidesmus indicus (Linn.) R.Br., its extracts and marketed formulations
    Journal:JPR:BioMedRx:An International Journal
    Vol(issue):1 (February)
    Year:2013
    Page No: (125-129)
  • Experimental Methods Keywords

    Methodology:HPTLC; Quantitation; Densitometric evaluation; Validation; Peak area.
    Research Materials:Hemidesmus indicus (Linn.) R.Br.

Keywords

Hemidesmus indicus Isovanillin  Formulations; HPTLC; Quantitation; Densitometric evaluation; Validation; Peak area.

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