Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002554
Title: Assessment of urease activity in soya bean products
Category: Pharmacology
Section: Research Article
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    Introduction: The present study is undertaken to assess the urease activity in soybean products. Materials and Methods: Soybean was taken and soaked overnight; it was then ground into a paste. The assay of urease was performed as described by Sharma et al. the enzyme was concentrated by precipitation followed by dialysis. Characterization of the enzyme was carried out by measuring its optimum pH and temperature. The molecular weight of the enzyme was analyzed by sodium dodecyl sulfate page. Result: In this study, the urease extracted from soybean was found to have an optimum temperature of 40°C, optimum pH of 7.0 and molecular weight by electrophoresis is 90kDa. Conclusion: Various plant sources contain urease; it is very economical to obtain and extract the enzyme from such sources. The extracted and purified urease can be used in the field of medicine and dentistry to counteract the action of the urease secreted by microorganisms causing rheumatoid arthritis, atherosclerosis, and dental caries, respectively. Future study and technology can be used to further extract the enzyme with a high degree of purity and use it in the field of medicine and dentistry.

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    Author(s) Name:

    S. Harrita1 , V. Vishnu Priya2 *, R. Gayathri 2

    Affiliation(s) Name:

    1 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India,

    2 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: V. Vishnu Priya, Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

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    Author:

    S. Harrita1 , V. Vishnu Priya2 *, R. Gayathri 2

    Title:Assessment of urease activity in soya bean products
    Journal:Drug Invention Today
    Vol(issue):10 (Oct)
    Year:2018
    Page No: (2045-2047)
  • Experimental Methods Keywords

    Methodology:Assay of Urease, Estimation of Protein, Characterization of the Enzyme
    Research Materials:Soybean

Keywords

Molecular weight Optimum temperature pH SDC-PAGE Soybean Urease activity

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