Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002662
Title: Evaluation of microbial contamination in stored anatomy specimens
Category: Pharmacology
Section: Research Article
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    Introduction: Dried bone specimens are used to teach osteology in anatomy laboratories. These bones are first boiled, made free from soft tissues and then dried and coated with turpentine. A microorganism is a microscopic organism, which may exist in its single-celled form or a colony of cells. Microorganisms that can cause infections from the contaminated bone specimens include bacteria, fungi, and viruses. These dried bone specimens are handled by the students and faculty for learning, so there is a need to check for microbial contamination present in the bone specimens to reduce the chances of infections among the students. Materials and Methods: The estimates sample size of 10 was chosen, and sample was collected aseptically by rotating sterile cotton swabs moistened with peptide water over the dried bone specimens used for teaching in the anatomy laboratory. The swabs are then cultured in nutritional agar medium and Sabouraud Dextrose Agar and incubated at 37°C for 24 h. The growth on the plates was differentiated and identified by morphology and Gram staining. Results: From the study, it was found that although there was no fungal contamination in the bone specimen, there was bacterial contamination found to be present in a few of the bone specimens. Conclusion: Not all the dried bone specimens are free from contaminations, a few bones have shown microbial contamination. Micrococcus occurs in a wide range in water, dust, soil, etc. The predominant method to prevent the cross infection is by practicing adequate hygiene practice. Hence, it is very important to maintain proper sterility of the dried bone specimens used for teaching anatomy so that there is no spread of cross infections in the future.

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    Author(s) Name:

    M. Neha Sharma1 , K. Yuvaraj Babu2 *, R. V. Geetha3

    Affiliation(s) Name:

    1 Department of Anatomy, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India,

    2 Department of Anatomy, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India,

    3 Department of Microbiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: K. Yuvraj Babu, Department of Anatomy, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

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    Author:

    M. Neha Sharma1 , K. Yuvaraj Babu2 *, R. V. Geetha3

    Title:Evaluation of microbial contamination in stored anatomy specimens
    Journal:Drug Invention Today
    Vol(issue):10 (December)
    Year:2018
    Page No: (2450-2452)
  • Experimental Methods Keywords

    Methodology: Contamination, Fungus, Infection, Microorganism
    Research Materials:Bacteria

Keywords

Bacteria Bones Contamination Fungus Infection Microorganism

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