Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002745
Title: In vitro evaluation of the inhibitory effect of lutein from marigold flower on sorbitol accumulation
Category: Pharmacology
Section: Research Article
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    Introduction: In diabetic state, the slow and steady activation of sorbitol pathway results in augmented accumulation of intracellular sorbitol which ultimately ends in diabetic complications. By preventing the intracellular accumulation of sorbitol, diabetic-related complications can be averted. The aim of the present study was to evaluate the effect of lutein from marigold flowers on sorbitol accumulation. Materials and Methods: Sorbitol accumulation in the presence of lutein was evaluated by fluorescent spectrophotometry. Heparinized rat blood was used for the study. 200 μl of washed erythrocytes was added to 1 ml of Hank’s balanced salt solution containing various concentrations of lutein or ascorbic acid (5–100 μg/ml). The reaction mixture contained the appropriate protein-free supernatant, 50 mM glycine buffer, 0.2 mM NAD+, and 1.28 units of sorbitol dehydrogenase. The mixture was incubated at 37°C for 30 min, and the relative fluorescence due to NADH was measured by a fluorescence spectrometer at an excitation wavelength of 366 nm and an emission wavelength of 452 nm. The experiments were performed in triplicates. Results: In the present study, lutein inhibited the sorbitol accumulation with maximum percentage inhibition of 81.58 ± 0.51% at 100 µg/ml. Standard ascorbic acid showed maximum percentage of 84 ± 0.68% at similar concentration. The half maximal inhibitory concentration value of lutein against sorbitol accumulation was found to be 38.39 µg/ml and that of the ascorbic acid was 37.37 µg/ml. Conclusion: Prevention of intracellular sorbitol accumulation is a widely recommended concept to avert diabetes-related complications. Hence, lutein may be used judiciously for the control of diabetic complications.

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    Author(s) Name:

    V. Balaji 1 , Anitha Roy2 *, T. Lakshmi 1

    Affiliation(s) Name:

    1 Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India,

    2 Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

    *Corresponding author: Dr. Anitha Roy, Associate Professor, Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, 162, Poonamalle High Road, Velapanchavadi, Chennai - 600 077, Tamil Nadu, India

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    Author:

    V. Balaji 1 , Anitha Roy2 *, T. Lakshmi 1

    Title:In vitro evaluation of the inhibitory effect of lutein from marigold flower on sorbitol accumulation
    Journal:Drug Invention Today
    Vol(issue):10 (Sept [ Special Issue 1 ])
    Year:2018
    Page No: (2809-2812)
  • Experimental Methods Keywords

    Methodology:In vitro evaluation,Diabetic complication
    Research Materials:Lutein

Keywords

Diabetic complication Lutein Marigold Sorbitol accumulation

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