Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002797
Title: Phytochemical screening and cytotoxic effect of methanolic leaf extract of Rhizophora apiculata blume against Michigan Cancer Foundation-7 breast cancer cell lines
Category: Pharmacology
Section: Research Article
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    Introduction: Rhizophora apiculata is an important traditional medicinal plant used in many people in Asia and Africa for curing of both infectious and non-infectious diseases. Objective: The present study was aimed to evaluate the cytotoxic effect and observation of cell morphology changes of breast cancer (Michigan Cancer Foundation-7 [MCF-7]) cell lines of after the treatment with the methanolic leaf extract of R. apiculata. Materials and Methods: Cytotoxicity of the methanolic leaf extract of R. apiculata was tested against MCF-7 cell lines by using MTT assay and to find the cell morphology of MCF- 7 cells upon exposure to the methanolic leaf extract of R. apiculata by using phase contrast microscope Results: The results showed that the methanolic leaf extract of R. apiculata with different concentrations (25,50, 100, and 200 μg/mL) had shown significant concentration-dependent anticancer (cytotoxic) activity against MCF-7 breast cancer cell by showing 48.27% of cell viability with half maximal inhibitory concentration (IC50 ) values are of 140.97 µg/ml, comparing with standard tamoxifen by showing 80.80% of cell viability with IC50 values of 61.88 µg/ml. Conclusion: From this study, it can be concluded that the methanolic leave extract of R. apiculata could be serve as a potential source of plant-derived, anticancer agents for the development of therapeutic anticancer drugs and further study has been focused on the isolation of bioactive anticancer compounds present in the methanolic leaf extract of R. apiculata by bioactivity-guided fractionation.

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    Author(s) Name:

    M. Muthulingam1 *, K. Krishna Chaithanya2

    Affiliation(s) Name:

    1Department of Zoology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India,

    2 Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Aksum, Ethiopia

    *Corresponding author: Dr. M. Muthulingam, Department of Zoology, Annamalai University, Annamalainagar - 608 002, Tamil Nadu, India

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    Author:

    M. Muthulingam1 *, K. Krishna Chaithanya2

    Title:Phytochemical screening and cytotoxic effect of methanolic leaf extract of Rhizophora apiculata blume against Michigan Cancer Foundation-7 breast cancer cell lines
    Journal:Drug Invention Today
    Vol(issue):10 (October [ Special Issue 2 ])
    Year:2018
    Page No: (2995-2998)
  • Experimental Methods Keywords

    Methodology:Michigan cancer foundation-7, Cytotoxicity, Cell morphology, 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay, Anticancer agents
    Research Materials: Rhizophora apiculata

Keywords

Rhizophora apiculata Michigan cancer foundation-7 Cytotoxicity Cell morphology 3-(4 5-dimethylthiazol- 2-yl)-2 5-diphenyltetrazolium bromide assay Anticancer agents

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