Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002801
Title: Confocal calcium imaging to study the molecular pharmacology of methacholine and atropine on muscarinic receptors
Category: Pharmacology
Section: Research Article
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    Introduction: Many studies are studying the pharmacology of important ligands on different receptors. Cholinergic receptors are one of the interested fields to study; as it is connected to many diseases and particularly Parkinson and Alzheimer. Objective: The aim of the research is to study the molecular pharmacology of atropine and methacholine on muscarinic acetylcholine receptors using confocal microscope to measure Ca level. Moreover, atropine and methacholine are used to study their molecular pharmacology on cholinergic receptors and especially muscarinic one on neuroblastoma cells (SH- SY5Y). Materials and Methods: The method was designed to treat SH-SY5Y cells with two different ligands (methacholine and atropine) separately, and then, atropine was added before loaded the cells with methacholine. Results: The results are as follows: Methacholine shows agonist effect with high potency while atropine is very controversial because it can be explained as either allosteric modulator or non-competitive antagonist in addition to its already approved competitive mechanism. Conclusion: In conclusion, atropine can be investigated for more pharmacological mechanisms which can help in different pathological conditions and specifically in neurological disorder.

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    Author(s) Name:

    Ali Jabbar Abdulhussein*

    Affiliation(s) Name:

    Department of Pharmacology and Toxicology, University of Baghdad, College of Pharmacy, Baghdad, Iraq

    *Correspondent author: Ali Jabbar Abdulhussein, Department of Pharmacology and Toxicology, University of Baghdad, College of Pharmacy, Baghdad, Iraq.

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    Author:

    Ali Jabbar Abdulhussein*

    Title:Confocal calcium imaging to study the molecular pharmacology of methacholine and atropine on muscarinic receptors
    Journal:Drug Invention Today
    Vol(issue):10 (October [ Special Issue 2 ])
    Year:2018
    Page No: (3012-3016)
  • Experimental Methods Keywords

    Methodology: Pharmacology,Confocal microscope, Muscarinic acetylcholine receptors
    Research Materials:methacholine and atropine

Keywords

Atropine Calcium level Confocal microscope Muscarinic acetylcholine receptors Methacholine Neuroblastoma cells

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