Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002872
Title: Antiproliferative activity of isolated bioactive flavonoid apigenin-7-O-β-D-glucuronide methyl ester from ethyl acetate leaf extract of Manilkara zapota
Category: Pharmacology
Section: Research Article
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    Introduction: Cancer is one of the major causes of death among the world, demonstrating a substantial public health problem. Medicinal plants have been a great source of secondary metabolites with antiproliferative activities. Apigenin- 7-O-β-D-glucuronide methyl ester is a bioactive flavonoid and has been isolated from the ethyl acetate leaf extract of Manilkara zapota, shown in vitro antiproliferative activities. Objective: The present study was aimed to evaluate the antiproliferative activity of bioactive flavonoid apigenin-7-O-β-D-glucuronide methyl ester against cancer cell lines such as human breast cancer (MCF-7), human hepatocellular carcinoma HEP G2, and human colon cancer cell lines (HT-29). Materials and Methods: Antiproliferative activity of apigenin-7-O-β-D-glucuronide methyl ester was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow tetrazolium assay. Results: The results showed that the apigenin-7-O-β-D-glucuronide methyl ester (1, 5, 10, 50, and 100 μg/mL) has exhibited significant shown a concentration- dependent moderate anticancer activities against HT-29 (colon cancer cell line) and MCF-7 breast cancer cell with half maximal inhibitory concentration (IC50) values are of 42.09 µg/ml and 40.17 µg/ml comparing with standards 5-fluorouracil and tamoxifen with IC50 values of 8.17 µg/ml and 7.72 µg/ml. Apigenin-7-O-β-D-glucuronide methyl ester shown strongest antiproliferative activity against HEP G2 (liver cancer cell) with IC50 value of 38.92 µg/ml compared with standard paclitaxel with IC50 values of 7.72 µg/ml. Conclusion: From this study, it can be concluded that the apigenin-7-O-β-D-glucuronide methyl ester exhibited significant anticancer activity on HEP G2 (liver cancer cell) and could serve as a potential source of plant derived, anticancer agents for the development of therapeutic anticancer drugs

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    Author(s) Name:

    K. Kamalakararao1,D. Govinda Rao2,M. Muthulingam3 , V. K. Gopalakrishnan4 , Zenebe Hagos4 , P. John Dogulas5 , K. Krishna Chaithanya4 *

    Affiliation(s) Name:

    1 Department of Biochemistry, Adikavi Nannaya University, Rajahmundry, Andhra Pradesh, India,

    2 Department of Biochemistry and Molecular Biology, School of Biological Sciences, Central University of Kerala, India,

    3 Department of Zoology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India,

    4 Departments of Chemistry, College of Natural and Computational Sciences, Aksum University, Aksum, Ethiopia,

    5 Departmentsof Life Sciences, University of Guanajuato, Guanajuato, Mexico

    *Corresponding author: Dr. Krishna Chaithanya Karri, Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Tigray Region, Post Box No 1010. Ethiopia.

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    Author:

    K. Kamalakararao1,D. Govinda Rao2,M. Muthulingam3 , V. K. Gopalakrishnan4 , Zenebe Hagos4 , P. John Dogulas5 , K. Krishna Chaithanya4 *

    Title:Antiproliferative activity of isolated bioactive flavonoid apigenin-7-O-β-D-glucuronide methyl ester from ethyl acetate leaf extract of Manilkara zapota
    Journal:Drug Invention Today
    Vol(issue):10 (November [ Special Issue 3 ])
    Year:2018
    Page No: (3352-3358)
  • Experimental Methods Keywords

    Methodology:Antiproliferative activity
    Research Materials: Manilkara zapota

Keywords

Antiproliferative activity Apigenin-7-O-β-D-glucuronide methyl ester HEP G2 HT-29 Manilkara zapota MCF-7

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