Journal: Drug Invention Today

Article Id: JPRS-Pcol-00002919
Title: Collagenase gel: Effect on wound healing processes in the experiment
Category: Pharmacology
Section: Research Article
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    Introduction: Collagen is one of the key targets of dermatotropic drugs. In this regard, the collagenase-based gel requires a deep preclinical study. Materials and Methods: Experiments were performed on 36 male rats. Using a blade, a linear wound length of 50 mm was modeled. Animals were divided into four groups: (1) Control, (2) collagenase (500 mg of gel with collagenase per wound area for 7 days), (3) Actovegin (500 mg of Actovegin gel on the wound area for 7 days), and (4) Contractubex (500 mg of Contractubex gel for 7 days wound area). About the wound healing effect of drugs was judged by studying the physicomechanical properties, determining the concentration of hydroxyproline (HP) and calculating the ratio of collagen 1 and 3 types. Results: The greatest strength of the wound defect, significantly higher than in the control (P < 0.01), was observed in the group that received the gel Actovegin (12.60 ± 0.63 N, P < 0.05). Significantly lower (P < 0.01) than in the control strength was obtained in the group using Contractubex (9.65 ± 0.59 N). The lowest strength (7.04 ± 0.55), significantly lower than in the control group (P < 0.01), characterized skin flaps obtained from animals treated with collagenase gel. During calorimetric analysis, it was found that the highest concentration of HP contained in the tissues of wound defects in animals treated with Contractubex; however, no statistically significant difference was found with the control group. Tissue from simulated wounds in animals treated with collagenase gel (73.4% of the control) contained a significantly lower concentration of HP compared with the control (P < 0.05). When assessing the ratio of collagen I and III in the tissues of the wound defect when dyeing with picrosirius red, it was found that by the number of mature collagen fibers, the studied groups can be arranged in the following sequence (descending): Actovegin> Collagenase> Control> Contractubex. Conclusions: Despite the demonstrated negative effects, the ability of cocollagenase to reduce the concentration of РЗ, increasing the relative amount of mature collagen, allows us to recommend this drug for further preclinical studies on models of scar defects.

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    Author(s) Name:

    Alena V. Majorova, Boris B. Sysuev, Ekaterina V. Tret’yakova, Vladislava A. Veselova, Vladislav O. Soldatov*, Olga A. Efremova

    Affiliation(s) Name:

    Department of Pharmacology and Clinical Pharmacology, Belgorod National Research University, 85 Pobedy st., Belgorod, 308015, Russia

    *Corresponding author: Vladislav O. Soldatov, Department of Pharmacology and Clinical Pharmacology, Belgorod National Research University, Russia.

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    Author:

    Alena V. Majorova, Boris B. Sysuev, Ekaterina V. Tret’yakova, Vladislava A. Veselova, Vladislav O. Soldatov*, Olga A. Efremova

    Title:Collagenase gel: Effect on wound healing processes in the experiment
    Journal:Drug Invention Today
    Vol(issue):10 (December [ Special Issue 4 ])
    Year:2018
    Page No: (3573-3577)
  • Experimental Methods Keywords

    Methodology:Wound healing processes
    Research Materials:Collagenase

Keywords

Collagenase Linear wound Reparation Rats

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