Journal: Drug Invention Today

Article Id: JPRS-BRPT-00003014
Title: Clustered regularly interspaced short palindromic repeats‑associated protein 9: A new era in targeted molecular therapy
Category: Biotechnology & Related Pharmaceutical Technology
Section: Research Article
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    In molecular biology to understand the critical role/s played by certain gene products, the encoding genes are often disrupted, and the effects are further analyzed to ascertain the exact function of the gene/protein in the development of the organisms. Recently emerged gene editing technique revolutionized the field of biology to study the pathology of various diseases and in the discovery of drugs. Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR-Cas9) attracted the attention of the World due to its impeccable potential in research and therapeutic applications. Unlike other techniques, CRISPR can deactivate a gene without interfering with intracellular mechanisms. It can be used to understand the genetic and epigenetic aspects of various diseases especially cancer. CRISPR can be used to induce specific mutations in oncogenes and tumor suppressor genes in cell lines to model the cancers to understand the pathology and to develop effective drugs. CRISPR-Cas9 holds the promising potential to treat genetic diseases such as Duchenne muscular dystrophy, Hemophilia, β-Thalassemia, and cystic fibrosis. However, there are some challenges and concerns which needs to addressed. In this review, we discuss on the origin of CRISPR-Cas9 technology, how it works, genome/nongenome editing for targeted molecular therapy and future directions.

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    Author(s) Name:

    K. Vishnupriyan, S. Ohm Prakash, M. Kavitha*

    Affiliation(s) Name:

    Department of Biomedical Sciences, School of Biosciences and Technology, Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India

    *Corresponding author: M. Kavitha, School of Biosciences and Technology, Vellore Institute of Technology, Vellore - 632 014, Tamil Nadu, India

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    Author:

    K. Vishnupriyan, S. Ohm Prakash, M. Kavitha*

    Title:Clustered regularly interspaced short palindromic repeats‑associated protein 9: A new era in targeted molecular therapy
    Journal:Drug Invention Today
    Vol(issue):11 (January)
    Year:2019
    Page No: (184-188)
  • Experimental Methods Keywords

    Methodology:Cancer, Clustered regularly interspaced short palindromic repeats, Genetic disorders, Genome editing, Imaging
    Research Materials:CRISPR associated protein 9

Keywords

CRISPR associated protein 9 Cancer Clustered regularly interspaced short palindromic repeats Genetic disorders Genome editing Imaging

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