Journal: Drug Invention Today

Article Id: JPRS-P col-00003030
Title: Evaluation of in vitro antibacterial potential of Eichhornia crassipes leaf extracts
Category: Pharmacology
Section: Research Article
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    Introduction: Eichhornia crassipes (water hyacinth) is a aquatic plant belonging to the family Pontederiaceae, traditionally it has been used as preparation of crude medicine in Ethiopia for curing various types of infectious disease. In developing countries, emerging of antibiotic resistance among bacterial pathogens brings the most serious public health challenge at a global scale. Objective: The aim of the present study was to determine the yield of crude extracts, phytochemical (secondary metabolites) screening of E. crassipes leaves using organic solvents ethanol and methanol and investigate the in vitro antibacterial activities against the tested bacteria. Materials and Methods: About 30 g of the ground leaves of E. crassipes were separately soaked in 150 ml of different solvents (ethanol and methanol) and left on a platform shaker for about 72 h and filtered using Whitman No.1 filter paper. The filtrates were then transferred into bottles, allowed for drying at room temperature for 5 days, and the antimicrobial activity of the extracts was evaluated against both Gram-positive and Gram- negative bacteria such as Staphylococcus aureus, Escherichia coli O157:H7, Salmonella typhi, and Shigella boydii using the disk diffusion method and determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration using broth dilution methods. Results: The results of the present study revealed that the ethanolic leaf extract of E. crassipes shown broadspectrum of antibacterial activity against tested Gram-positive and Gram-negative bacteria. The ethanolic leaf extract of Eichhornia had shown significant antibacterial activity against S. aureus with lowest MIC of 25 mg/ml and 50 mg/ml minimum bacterial concentration due to the presence of secondary metabolites such as alkaloids, tannins, saponins, terpenoids, and steroids. Conclusion: From this study, it can be concluded that the ethanolic leaf extract of E. crassipes could be served as a potential source of natural antibacterial agents for the development of therapeutic antibiotics helpful in to treat the community and hospital-acquired infectious diseases.

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    Author(s) Name:

    Tensay G. Kiristos1 , Ameha Kebede2 , K. Krishna Chaithanya3 , M. Zenebe Teka1 *

    Affiliation(s) Name:

    1 Department of Biology, College of Natural and Computational Sciences, Aksum University, Aksum, Ethiopia,

    2 Department of Biology, School of Biological Science and Biotechnology, Haramaya University, Oromia, Ethiopia,

    3 Departments of Chemistry, College of Natural and Computational Sciences, Aksum University, Aksum, Ethiopia

    *Corresponding author: Dr. Zenebe Teka Mengesha,Department of Biology College of Natural and Computational Sciences, Aksum University, Axum, Tigray Region, Post Box No. 1010, Ethiopia

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    Author:

    Tensay G. Kiristos1 , Ameha Kebede2 , K. Krishna Chaithanya3 , M. Zenebe Teka1 *

    Title:Evaluation of in vitro antibacterial potential of Eichhornia crassipes leaf extracts
    Journal:Drug Invention Today
    Vol(issue):10 (December [ Special Issue 5 ])
    Year:2018
    Page No: (8324-8331)
  • Experimental Methods Keywords

    Methodology:Agar well diffusion, Antibacterial activity, Eichhornia crassipes, Minimum inhibitory concentration
    Research Materials: Eichhornia crassipes leaf

Keywords

Agar well diffusion Antibacterial activity Eichhornia crassipes Minimum inhibitory concentration

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