Journal: JPR:BioMedRx:An International Journal

Article Id: JPRS-DVD-0000305
Title: Validated HPLC Method Development for Simultaneous Estimation of Paracetmol and Flupiritine in bulk drug and in formulation dosage forms
Category: Development and Validation of Drugs
Section: Research Article
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    A simple, sensitive, accurate and precise RPHPLC method for the simultaneous determination of paracetmol and flupiritine in a bulk drug and pharmaceutical formulations was developed. . Chromatographic separation was achieved on thermo hypersil BDS column, with phosphate buffer- methanol (50:50v/v, pH, 2.3) as mobile phase at flow rate of 1.0mL/min.Quantification was achieved with PDA detector at 250nm.The retention time for paracetmol and Flupiritine was found at 3.4 and 5.4minutes respectively. The linearity for both the drugs were observed in the concentration range of 50-150µg/ml with mean accuracies 98%-101%. The method was successfully applied to pharmaceutical formulation because of no chromatographic interferences from blank and placebo were found. The method retains its accuracy and precision when the standard addition technique was applied.

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    Author(s) Name:

    Gopisetty Nagamani and Katreddi Hussain Reddy

    Affiliation(s) Name:

    Department of Chemistry, Sri Krishnadevaraya University Ananthapur- 515 003, Andhra Pradesh, India.

    *Corresponding author.
    Gopisetty Nagamani
    Department of Chemistry,
    Sri Krishnadevaraya University
    Ananthapur- 515 003,
    Andhra Pradesh, India.


    Received on:11-01-2013; Revised on: 07-02-2013; Accepted on:25-03-2013

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    Author:

    Gopisetty Nagamani and Katreddi Hussain Reddy

    Title:Validated HPLC Method Development for Simultaneous Estimation of Paracetmol and Flupiritine in bulk drug and in formulation dosage forms
    Journal:JPR:BioMedRx:An International Journal
    Vol(issue):1 (April)
    Year:2013
    Page No: (402-405)
  • Experimental Methods Keywords

    Methodology:HPLC Analysis
    Research Materials:Paracetmol, flupiritine

Keywords

Paracetmol flupiritine High performance chromatography (HPLC) Simultaneous determination

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