Journal: Drug Invention Today

Article Id: JPRS-P col-00003059
Title: Comparative evaluation of antimicrobial activity of 3% sodium hypochlorite, 2% chlorhexidine, and 5% grape seed extract against Enterococcus faecalis and Candida albicans - An in vitro study
Category: Pharmaceutics
Section: Research Article
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    Aim: This study aims to compare the antimicrobial efficacy of different irrigants such as 3% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate, and 5% grape seed extract against Enterococcus faecalis and Candida albicans. Background: Debridement and disinfection of the root canal system is a critical step in endodontic treatment. Most of the irrigants presently used in the endodontic treatment can have an impact on the microbes surviving in the biofilm, but none of them are able to do all of the required tasks. Researches are going on its full swing to produce an endodontic irrigant having ideal properties; for many years, intracanal irrigants have been used as an adjunct to enhance antimicrobial effect of cleaning and shaping in endodontics. The constant increase in antibiotic-resistant strains and side effects of synthetic drugs has promoted researchers to look for herbal alternatives. For thousands of years, humans have sought to fortify their health and cure various illnesses with herbal remedies, but only few have been tried and tested to withstand modern scientific scrutiny. The present study was aimed to evaluate alternative and inexpensive simple and effective means of sanitization of the root canal systems. The antimicrobial efficacy of herbal alternatives as endodontic irrigants is evaluated and compared with the standard irrigants 3% NaOCl and 2% chlorhexidine. Materials and Methods: The irrigation solution was divided into three groups: Group I - 3% sodium hypochlorite, Group II - 2% chlorhexidine, and Group III - 5% grape seed extract. The zones of inhibition of growth were recorded against E. faecalis and C. albicans. Strains of these microorganisms were isolated and were subcultured. Disc diffusion was done for the primary evaluation of antimicrobial susceptibility, in which microorganisms were incubated in agar plates, following which 50 µL of each irrigant was added in sterile disc and placed on the plates. The plates were incubated and zone of inhibition around each disc was measured. Results: About 5% grape seed extract showed zones of inhibition, suggesting that they had antimicrobial properties. 5% grape seed extracts showed significantly greater zones of inhibition than 2% chlorhexidine. Henceforth, research should be directed toward the use as an irrigant in root canal treatment. Conclusion: Under the limitations of this study, it was concluded that 5% grape seed extract has a significant antimicrobial effect against E. faecalis. Microbial inhibition potential of grape seed extract observed in this study opens perspectives for its use as an intracanal irrigant.

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    Author(s) Name:

    S. Arun Kumar1 , P. Ajitha1 *, Raghu Sandhya1 , Muralidaran2

    Affiliation(s) Name:

    1 Department of Conservative Dentistry and Endodontics, Saveetha Dental College, Saveetha University, Chennai, Tamil Nadu, India,

    2Department of Microbiology, Saveetha Dental College, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: Dr. P. Ajitha, Department of Conservative Dentistry and Endodontics, Saveetha Dental College, Saveetha University, No 162, Poonamallee High Road, Chennai – 600 077, Tamil Nadu, India

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    Author:

    S. Arun Kumar1 , P. Ajitha1 *, Raghu Sandhya1 , Muralidaran2

    Title:Comparative evaluation of antimicrobial activity of 3% sodium hypochlorite, 2% chlorhexidine, and 5% grape seed extract against Enterococcus faecalis and Candida albicans - An in vitro study
    Journal:Drug Invention Today
    Vol(issue):12 (January)
    Year:2019
    Page No: (53-56)
  • Experimental Methods Keywords

    Methodology:antimicrobial activity, Well diffusion method
    Research Materials:Grape seed extract

Keywords

Candida albicans Endodontic irrigants Enterococcus faecalis Grape seed extract Well diffusion method

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