Journal: Drug Invention Today

Article Id: JPRS-P col-00003060
Title: High GSK3β expression in Pkd1 cells
Category: Pharmaceutics
Section: Research Article
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    Background: Polycystic kidney disease (PKD) is an inherited disease characterized by renal cysts and progressive decline in renal function. At the molecular level, PKD thought to be caused by defective primary cilia structure and/or function. Renal cells showed increased proliferation profile leading to progressively enlarged cysts. Primary cilia, as the mechanosensory organelles sensitize fluid flow and trigger intracellular events. Regulation of cell proliferation is one of the cellular functions regulated by ciliary signaling. GSK3β, a protein kinase that play a vital role in cell proliferation. Methods: In this study, analysis of the GSK3β and p-GSK3β protein expression by western blot in renal mouse Pkd1 cells was performed as a prospected pathogenic pathway of renal cyst. Results: Data revealed that GSK3β expression in Pkd1 cells significantly higher than wild type cells proposing a high proliferative state of cell. In the same manner, p-GSK3β, the inactive form, showed to be significantly low in Pkd1 cells compared to wild type renal cells. Conclusion: Pkd1 cells showed high GSK3β and low p-GSK3β protein expression, implying a high proliferative profile of cells and suggest a mechanism for renal cyst expansion.

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    Author(s) Name:

    Sarmed H. Kathem*

    Affiliation(s) Name:

    Department Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Babalmoadhum 14026, Baghdad, Iraq

    *Corresponding author: Sarmed H. Kathem, Department Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Babalmoadhum 14026, Baghdad, Iraq

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    Author:

    Sarmed H. Kathem*

    Title:High GSK3β expression in Pkd1 cells
    Journal:Drug Invention Today
    Vol(issue):12 (January)
    Year:2019
    Page No: (57-59)
  • Experimental Methods Keywords

    Methodology:Cilia bending, Primary cilia, Renal cyst
    Research Materials: Polycystic kidney disease (PKD

Keywords

Cilia bending Primary cilia Renal cyst

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