Journal: Drug Invention Today

Article Id: JPRS-P col-00003062
Title: Fluid flow generates delayed calcium current in renal epithelial cells in vitro
Category: Pharmaceutics
Section: Research Article
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    Background: Primary cilium is the mechanosensor of the cell protruding from the apical side of almost all cells in the body. Primary cilia paly crucial role in regulating various cellular functions, while dysfunctional cilia play an important role in the pathogenesis of many diseases collectively known as ciliopathy. Due it cilium central role, it is vital to study what signal it generate to coordinate these events. Methods: In this experiment, renal epithelial cells used to study the signaling events of primary cilia. Genetically encoded calcium indicator (GECI) was used for a precise measurement of calcium ion concentration in real time experiment. In this technique, a single cell live imaging method was utilized with a lateral view of the cell. Results: Data revealed that fluid flowing over the cells leads to cilium bending and generates calcium ion peak in the cytoplasm. The calcium ion concentration begin to start after 0.54 second and peak concentration reported in 0.85 seconds after flow start. Depleting the medium and the flowing fluid from calcium by EGTA abolishes calcium peak. Conclusion: Renal epithelia cells respond to fluid flow by generating delayed calcium current and is dependent on extracellular calcium entry to cell.

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    Author(s) Name:

    Sarmed H. Kathem*

    Affiliation(s) Name:

    Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Babalmoadhum 14026, Baghdad, Iraq

    *Corresponding author: Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Babalmoadhum 14026, Baghdad, Iraq.

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    Author:

    Sarmed H. Kathem*

    Title:Fluid flow generates delayed calcium current in renal epithelial cells in vitro
    Journal:Drug Invention Today
    Vol(issue):12 (January)
    Year:2019
    Page No: (63-66)
  • Experimental Methods Keywords

    Methodology:Measurement of Calcium Ion,Calcium Ion Concentration in Response to Flow
    Research Materials:renal epithelial cells

Keywords

Cilia bending Primary cilia Renal cyst

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