Journal: Drug Invention Today

Article Id: JPRS-P col-00003156
Title: Anticancer activity of defense peptides from Galleria mellonella on cervical hela cancer cell line
Category: Pharmaceutics
Section: Research Article
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    Aim: The statistical report shows that cervical cancer is the fourth most common neoplasm in women. Thus, the productions of natural drugs with good anticancer properties are highly necessitated. Several studies demonstrated the activities of Galleria mellonella in biological stream. The hemolymph peptides can exert immune properties in vitro and in vivo. Materials and Methods: Keeping all research reports as background, we have formulated the study of hemolymph and supernatant extracts efficacy on HeLa cell line with various concentrations of 10 µg/ml, 20 µg/ml, 30 µg/ml, 40 µg/ml, and 50 µg/ml, respectively. In in vivo, G. mellonella is used as model in the field of entomological life. These characteristics along with its availability as anticancer, antimicrobial, antifungal, and antileishmanial activities made the Galleria hemolymph promising seal in the research world. Results: The results revealed the effectiveness of G. mellonella hemolymph and supernatant extracts in repressing the growth of HeLa cells. Noticeable inhibition was recorded in all concentrations of hemolymph and supernatant extracts. The HeLa cell line treated with supernatant of G. mellonella concentrations of 10 µg/ml, 20 µg/ml, 30 µg/ml, 40 µg/ ml, and 50 µg/ml showed percentage inhibition apoptosis of 47.37 ± 1.92%, 49.7 ± 1.3%, 56.00 ±3.28%, 71.40 ± 3.9%, and 74.8 ± 2.6%, respectively. Moreover, the samples treated with hemolymph extracts concentrations of 10 µg/ml, 20 µg/ ml, 30 µg/ml, 40 µg/ml, and 50 µg/ml recorded highest percentage of the inhibition of 63.68 ± 2.3%, 76.20 ± 2.9%, 84.73 ± 3.5%, 97.09 ± 0.12%, and 97.30 ± 0.04%, respectively. The IC50 value of the Galleria hemolymph was found 2.3 µg/ ml and for Galleria supernatant 3.34 µg/ml, respectively. The molecular weight of the proteins present in hemolymph and the supernatant ranged between 28 KDa and 30 KDa compound and peak value of 5.282 was observed in hemolymph extracts in high-performance liquid chromatography analysis. Conclusion: To the best of our knowledge, it is the first report communicating investigation on maximum inhibition rate in HeLa cell line using the Galleria hemolymph extracts.

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    Author(s) Name:

    Jissin Mathew, C. Vani*, Amala Lizy

    Affiliation(s) Name:

    Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India


    *Corresponding author: C. Vani, Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore – 641 114 Tamil Nadu, India.

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    Author:

    Jissin Mathew, C. Vani*, Amala Lizy

    Title:Anticancer activity of defense peptides from Galleria mellonella on cervical hela cancer cell line
    Journal:Drug Invention Today
    Vol(issue):11 (February)
    Year:2019
    Page No: (442-447)
  • Experimental Methods Keywords

    Methodology:Anticancer, High-performance liquid chromatography, 3-(4, 5-dimethythiazol2-yl)-2,5- diphenyltetrazolium bromide assay, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Therapeutic effects
    Research Materials:Galleria mellonella

Keywords

Anticancer Galleria mellonella High-performance liquid chromatography 3-(4 5-dimethythiazol2-yl)-2 5- diphenyltetrazolium bromide assay Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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