Journal: Drug Invention Today

Article Id: JPRS-DFP-00003219
Title: Isolation and identification of Withaferin A (Steroidal Lactone) from Withania somnifera (Ashwagandha)
Category: Drugs from Plants
Section: Research Article
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    Background: This paper emphasizes a method for separation, identification, and quantification of selected withanolides in Withania somnifera plant extracts by HPLC estimation. Method: Withaferin-A which was isolated from Withania somnifera by repeated column chromatography of the Ashwagandha extract and their identity was established by 1H- and 13C-NMR was used as external standard. The compound was chromatographed on an isocratic system with a mobile phase made up of ACN: water 1:1 (v/v); flow of 0.6 mL/min; injection volume of 20 mL; λ = 225 nm; a LiChroCART 250×4 mm (5µm) column. The method was applied successfully to the detection and quantification of the said withanolide in the Ashwagandha extract. Result: On repeated chromatography over silica gel (100-200 Mesh Size), fraction no. 18-24 led to the isolation of compound one expected to be Withaferin A. Withaferin A was eluted out at 13.08 minute in HPLC run and further characterisation also confirms the compound. Conclusion: The method to separate, identifiy and characterize Withaferin-A from Withania somnifera was very much appropriate and reliable.

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    Author(s) Name:

    S. Madhu1 *, M. Komala1 , P. Pandian2

    Affiliation(s) Name:

    1 Department of Pharmacy, Faculty of Engineering and Technology, Chidambaram, Tamil Nadu, India,

    2 Department of Pharmacy, Faculty of Engineering and Technology, Annamalai University, Chidambaram, Tamil Nadu, India

    *Corresponding author: S. Madhu, Department of Pharmacy, Faculty of Engineering and Technology, Chidambaram, Tamil Nadu, India.

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    Author:

    S. Madhu1 *, M. Komala1 , P. Pandian2

    Title:Isolation and identification of Withaferin A (Steroidal Lactone) from Withania somnifera (Ashwagandha)
    Journal:Drug Invention Today
    Vol(issue):11 (March)
    Year:2019
    Page No: (595-600)
  • Experimental Methods Keywords

    Methodology:Fourier transform infrared, High-performance liquid chromatography, Nanoparticles
    Research Materials: Withaferin A, Withania somnifera

Keywords

Fourier transform infrared High-performance liquid chromatography Nanoparticles Withaferin A Withania somnifera

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