Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-0000322
Title: Method development and validation of RP-HPLC method for simultaneous determination of ibuprofen and methocarbamol
Category: Analytical Chemistry
Section: Research Article
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    A rapid, sensitive and specific RP-HPLC method involving UV detection was developed and validated for determination and quantification of Ibuprofen and Methocarbamol. Chromatography was carried out on a pre-packed Develosil, ODS-MG-5 (150*4.6mm, 5μ column using filtered and degassed mixture of chloroacetic acid buffer and acetonitrile in the ratio of (400:600) as mobile phase at a flow rate of 1.0ml/min and effluent was monitored at 230nm. The method was validated in terms of linearity, precision, accuracy, and specificity, limit of quantification and limit of detection. The assay was linear over the concentration range of Ibuprofen and Methocarbamol was 20mcg-60mcg/ml and 500mcg to 1500mcg/ml respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 99.7%-100.06% and 99.90%-100.01% within precision RSD of 0.05 and 0.94 for Ibuprofen and Methocarbamol respectively. The system suitability parameters such as theoretical plates and tailing factor were found to be 9610.4, 1.2 and 4611.8, 1.1 respectively for Ibuprofen and Methocarbamol. The method does require only 10 minutes as run time for analysis which prove the adoptability of the method for the routine quality control of the drug.

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    Author(s) Name:

    M.V.Kumudhavalli*, B.Jayakar, R.Margret Chandira, M.Kumar, C.Saravanan.

    Affiliation(s) Name:

    Department of Pharmaceutical Analysis, Vinayaka Mission’s College of Pharmacy,Vinayaka Missions University, Salem-636008, Tamilnadu.

    *Corresponding author.
    M.V.Kumudhavalli
    Department of Pharmaceutical Analysis,
    Vinayaka Mission’s College of Pharmacy,
    Vinayaka Missions University,
    Salem-636008, Tamilnadu.


    Received on: 28-09-2009; Revised on: 24-12-2009; Accepted on:14-02-2010

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    Author:

    M.V.Kumudhavalli*, B.Jayakar, R.Margret Chandira, M.Kumar, C.Saravanan.

    Title:Method development and validation of RP-HPLC method for simultaneous determination of ibuprofen and methocarbamol
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):1 (February)
    Year:2010
    Page No: (28-30)
  • Experimental Methods Keywords

    Methodology:RP-HPLC method
    Research Materials:ibuprofen and methocarbamol

Keywords

Ibuprofen Methocarbamol method development validation

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