Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-0000323
Title: Comparative study on identification and estimation of Methionine in the pharmaceutical dosage forms
Category: Analytical Chemistry
Section: Research Article
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    Aim: Identification and estimation of methionine in pharmaceutical Dosage forms by different Analytical methods. Methods :A novel method for the identification and estimation of DL-Methionine in various pharmaceutical dosage forms like tablets and syrups was developed. Identification was done by the Paper chromatography technique; identification carried out by measuring color development by ninhydrin as a spray reagent. The absorbance of the solution was measured at 570 nm. Beer-Lambert’s law was observed to obey in the range of 10-100 mcg. In the estimation of methionine in pharmaceutical dosage forms, the quadratic polynomial coefficient was computed by measuring the fluorescence of the drug in 0.01N hydrochloric acid and six points equally spaced at 5nm levels on the emission spectrum from 550 to 575 nm were plotted. The quadratic polynomial coefficient was found to be linear (directly proportional) to the concentration in the range 0.5 to 2.0mcg. Results: Recoveries were quantitative with good reproducibility. Excipients like bronidiol, parabens, essences, sugars and coloring agents did not interfere with proposed methods, Method A. (Beer-Lamberts Law ) both the isomers of Methionine D AND L forms gave Rf values of 0.32 and 0.40 respectively. The linear responses were in the range of 10-100mcg. The experimental recovery was 98-99% with 99-100% reproducibility.Method B: Paracetamol and Ibuprofen did not interfere with the proposed method. Estimation of Methionine by method B (orthogonal polynomials) was although linear but comparatively less precise with recoveries of 97-99% with 99% reproducibility. Conclusion: The above findings suggest that the estimation of the compound could be done by either of the methods with a good correlation between them within the specified limits of concentration. How ever comparatively Beer-Lamberts Law is found to be much more precise than the other. Thus, the analysis of compound by orthogonal polynomial coefficient although is an important and convenient tool, but less effective in comparison to other method (beer lamberts law).

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  • About the authors and Affiliations

    Author(s) Name:

    Mohammed Ibrahim*#, Syed Mohammed Kazim*, DK Vatsa# and GJ Rao#.

    Affiliation(s) Name:

    * Nizam Institute of Pharmacy, Deshmukhi (V), Pochampally (M), Nalgonda (D) – 508284
    # Smith and Kenner Pharmaceutical (Pvt) Limited, Hyderabad – 500 036

    *Corresponding author.
    Mohammed Ibrahim,
    Nizam Institute of Pharmacy, Deshmukhi,
    Pochampally (Mandal), Near Ramoji Film City, Nalgonda-508284, Andhra
    Pradesh, India.

    Received on: 20-09-2009; Revised on: 16-11-2009; Accepted on:19-01-2010

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    Mohammed Ibrahim*#, Syed Mohammed Kazim*, DK Vatsa# and GJ Rao#.

    Title:Comparative study on identification and estimation of Methionine in the pharmaceutical dosage forms
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):1 (February)
    Page No: (31-34)
  • Experimental Methods Keywords

    Methodology:quantitative analysis,
    Research Materials:Methionine


DL-Methionine; Paracetamol; Ibuprofen and Paper chromatography and orthogonal polynomials.

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