Journal: Drug Invention Today

Article Id: JPRS-P col-00003255
Title: Amino oxidase activities of antitumor enzyme producer from Trichoderma
Category: Pharmaceutics
Section: Research Article
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    Aim: The biosynthesis of L-lysine-α-oxidase enzyme by Trichoderma fungus has been studied at various cultivation methods and on the media with various carbon sources. In the work presented, various variants of media and the possibility of wheat bran and hemicellulose substrate replacement by other carbon sources in connection with an enzyme development were studied, as well as the substrate specificity of Trichoderma during the use of this substrate depending on the culture and acidity medium age. Materials and Methods: The vegetative mycelium of mushrooms, which was grown in 8-10 days at t - 28°C on the Chapek medium, served as a seed material. The study was carried out in a deep-seated manner using the laboratory shaker No. 357 (“Poland”) and a surface method of cultivation. Results: The enzyme was synthesized actively only on the medium with wheat bran. Trichoderma strains, when they were grown on the medium with a hemicellulose substrate, exhibited a different spectrum of L-amino acid oxidase activities. With the age of the Trichoderma culture, the spectrum of aminoxidase activities expanded, but it remained individual for each strain and could serve as a marker for its characteristics. Conclusion: As can be seen from the presented results, a specific spectrum of amino acid degradation manifests itself for each strain of various representatives of Trichoderma - the producers of L-lysine-a-oxidase, which can serve as a biochemical characteristic of the strain.

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    Author(s) Name:

    I. P. Smirnova*, O. M. Kuznetsova, A. S. Botin, S. P. Siatkin, V. I. Kuznetsov, G. I. Myandina, M. K. Nurmuradov

    Affiliation(s) Name:

    Department of Biochemistry, Medical Institute, Peoples’ Friendship University of Russia (RUDN University), Moscow, Russian Federation

    *Corresponding author: I. P. Smirnova, Department of Biochemistry, Medical Institute, Peoples’ Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya Street, Moscow, 117198, Russian Federation.

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    Author:

    I. P. Smirnova*, O. M. Kuznetsova, A. S. Botin, S. P. Siatkin, V. I. Kuznetsov, G. I. Myandina, M. K. Nurmuradov

    Title:Amino oxidase activities of antitumor enzyme producer from Trichoderma
    Journal:Drug Invention Today
    Vol(issue):11 (March)
    Year:2019
    Page No: (758-762)
  • Experimental Methods Keywords

    Methodology:Amino oxidase activities
    Research Materials:vegetative mycelium of mushrooms

Keywords

Amino oxidase Enzyme L-lysine-α-oxidase biosynthesis Trichoderma

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