Journal: Journal of Pharmacy Research

Article Id: JPRS-PA-0000336
Title: Analytical Method Development and Validation for the Estimation of Ursodeoxycholic Acid using RP-HPLC.
Category: Pharmaceutical Analysis
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    The Present work is undertaken with an aim to develop and validate an accurate, precise and rapid method for the estimation of Ursodeoxycholic Acid in tablet and bulk dosage form. The method is simple, accurate and sensitive. The separation was achieved on a gradient high pressure liquid chromatograph (Jasco, RI 2031 Plus) pumps Jasco PU 2080 Plus, IR detector column oven (Jasco), system controller (Jasco), a disposable guard column LC-18 (PELLIGUARD)TM , LC-18, 2 cm, supelco, inc., Bellefonte, and a Reverse Phase C-18 (phenyl) Column (25 cm x 4.6 mm) i.d., particle size 5 µm) was used The HPLC system was equipped with the software class, Borwin (Jasco). Flow Rate : 1.0 ml/min Injection Volume : 10µl Run Time : 15 min, Detector temp: 40 oC. The method was validated for specificity, precision, linearity, and accuracy. The recovery range for baclofen is in the range of 99.0–102.0% and the method can be successfully applied for the routine analysis of the drug substance.

    Cite this article as: Soni Varinder, Parminder Kumar, Saini Gurjeet,Gagan shah, R.K Dhawan,Analytical Method Development and Validation for the Estimation of Ursodeoxycholic Acid using RP-HPLC,Journal of Pharmacy Research 2015,9(1),46-53.

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    Author(s) Name:

    Soni Varinder*1, Parminder Kumar5, Saini Gurjeet2, Gagan shah3, R.K Dhawan4

    Affiliation(s) Name:

    *1 Department of Pharmaceutical chemistry, 2Pharmaceutics, 3Pharmacognosy and
    4Pharmacology Khalsa College of Pharmacy, Amritsar,India.
    5 Department of Pharmacy, B.I.S College of Pharmacy, Gagra-142043 (Moga) Punjab,India.

    *Corresponding author.
    Soni Varinder
    Department of Pharmaceutical chemistry,
    Khalsa College of Pharmacy, Amritsar,India.


    Received on: 12-08-2014; Revised on: 11-10-2014; Accepted on:18-12-2014

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    Author:

    Soni Varinder*1, Parminder Kumar5, Saini Gurjeet2, Gagan shah3, R.K Dhawan4

    Title:Analytical Method Development and Validation for the Estimation of Ursodeoxycholic Acid using RP-HPLC.
    Journal:Journal of Pharmacy Research
    Vol(issue):9(1)
    Year:2015
    Page No: (46-53)
  • Experimental Methods Keywords

    Methodology:RP-HPLC analysis
    Research Materials:Ursodeoxycholic Acid

Keywords

RP HPLC Baclofen Method Development UV Detector Validation

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