Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-CE-0000348
Title: Tannin acyl hydrolase from Trichoderma viride
Category: Chemical Engineering
Section: Research Article
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  • Abstract

    The enzyme tannase (tannin acyl hydrolase EC 3.1.1.20) produced from Trichoderma viride (MTCC 167), was purified and characterized. Optimal conditions for the production tannase were determined using varying substrate concentrations Fermentation was carried out for 48 h for optimum enzyme production and the properties of the enzyme were investigated. The optimum conditions of temperature and pH were investigated and the effects of nitrogen sources, metal ions and chelator were studied. Tannase exhibited optimum activity at 45°C and at pH of 5.5 urea concentrations higher than 3 M were inhibitory. Increasing concentrations of sodium also led to decrease in activity. Increasing concentrations of EDTA had an inhibitory effect on tannase. Among the nitrogen sources selected ammonium ferrous sulfate, ammonium sulfate, ammonium nitrate and ammonium chloride enhanced tannase activity at 0.1% (W/V) concentration.

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  • About the authors and Affiliations

    Author(s) Name:

    N. Lokeswari1, K.Jaya Raju1, Sudhakar Pola2 and Varaprasad Bobbarala3*

    Affiliation(s) Name:

    1Department of Chemical Engineering, AU College of Engineering, Andhra University, Visakhapatnam-3
    2Department of Biotechnology, AU College of Science and Technology, Andhra University, Visakhapatnam-3
    3Vivimed labs Limited, Veeranag towers, Habsiguda, Hyderabad, Andhra Pradesh.


    Received on: 21-02-2010; Revised on: 11-03-2010; Accepted on:14-04-2010

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    Author:

    N. Lokeswari1, K.Jaya Raju1, Sudhakar Pola2 and Varaprasad Bobbarala3*

    Title:Tannin acyl hydrolase from Trichoderma viride
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):1 (May)
    Year:2010
    Page No: (106-109)
  • Experimental Methods Keywords

    Methodology:Tannase assay, Fungal Tannase.
    Research Materials:Tannase

Keywords

Additives Tannase Tannase assay Fungal Tannase.

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