Journal: Drug Invention Today

Article Id: JPRS-BRPT-00003496
Title: Production and purification of β-galactosidase from Aspergillus foetidus MTCC 6322 using solid-state fermentation
Category: Biotechnology & Related Pharmaceutical Technology
Section: Research Article
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    Objective: β-galactosidase (E. C.3.1.2.23) otherwise known as lactase plays a vital role in food processing and pharmaceutical industries. The present study deals with the production of one enzyme β-galactosidase which could be useful for industrial implementation. This enzyme was produced from Aspergillus foetidus MTCC 6322 using solid-state fermentation. Materials and Methods: One strain of A. foetidus was isolated from tannery sludge and maintained at 4°C in Czapek-Dox agar slants. 100 µL of the spore suspension containing 6.0 × 107 CFU/ml was used as the seed inoculums. Fermentation was carried out in 250 mL Erlenmeyer flasks. 10 g of substrate in 250 mL Erlenmeyer flasks was moistened with 15.0 mL of distilled water and sterilized. After cooling, 1000 µL of A. foetidus spore suspension was added to the substrate and mixed thoroughly. They were incubated at 30°C for 96 h. Production of β-galactosidase was standardized by one-factor-at-a-time method. Results: The maximum amount of enzyme activity of 83.40/mg was observed when gingelly oil cake (7.5 g) and groundnut oil cake (2.5 g) containing 60% moisture and 1.5 ml of inoculums suspension were used at temperature 30°C and pH 4.0, respectively, after 96 h of incubation. Conclusions: Thus, it is clear that this process of obtaining the enzyme is feasible and it is suggested that this process can be implemented by the industry.

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    Author(s) Name:

    M. R. K. Rao*, T. V. Rishikesh

    Affiliation(s) Name:

    Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India

    *Corresponding author: Dr. M. R. K. Rao, Department of Industrial Biotechnology, Bharath Institute of Higher Education and Research, Chennai - 600 073, Tamil Nadu, India

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    Author:

    M. R. K. Rao*, T. V. Rishikesh

    Title:Production and purification of β-galactosidase from Aspergillus foetidus MTCC 6322 using solid-state fermentation
    Journal:Drug Invention Today
    Vol(issue):11 (May)
    Year:2019
    Page No: (1218-1219)
  • Experimental Methods Keywords

    Methodology:Production and purification
    Research Materials:Aspergillus foetidus MTCC 6322

Keywords

Agar Aspergillus foetidus Inoculation One-factor-at-a-time Solid-state fermentation β-galactosidase

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