Journal: Drug Invention Today

Article Id: JPRS-P col-00003517
Title: Shelf life of a refrigerated FNAC(Fine needle aspiration cytology) specimen
Category: Pharmacology
Section: Research Article
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    Introduction: Fine-needle aspiration cytology (FNAC) procedures may be used for rapid, cost effective, and apt diagnosis with reduced patient morbidity. Anatomical sites most commonly obtained from are breast, thyroid, lymph nodes, and deep soft tissue lesions. The FNAC specimen is usually refrigerated; in case, we need an additional smear or need to use additional stains for diagnosis. The tendency to refrigerate an FNAC specimen after smearing is quite usual, but it is important to know how long an FNAC will provide useful information if refrigerated. The aim of this study was to evaluate the usefulness of a refrigerated FNAC specimen. Materials and Methods: Five FNAC specimens were collected. They were smeared on alternative days till the 15th day. The smears were immediately fixed in alcohol. They were stained by routine hematoxylin and eosin protocol and mounted. The slides were viewed and scored by two observers. The scoring was based on the quality of information provided by the smear on the basis of cellular content, nuclear details, cytoplasmic details, uniformity of spread, and background effect on the diagnosis. Results: There was no significant change in the quality of information produced by the slides smeared on the 15th day, but there was evidence of clumping of cells after a week due to increased viscosity of the FNAC specimen. After a period of 1 month, there was microbial overload of the specimen. Conclusion: The FNAC specimens can be refrigerated for a period of 2 weeks to provide quality information, further which shows clumping of cells and microbial contamination.

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    Author(s) Name:

    P. K. Uma*, Pratibha Ramani, Herald. J. Sherlin, S. Gheena, Gifrina Jayaraj, K. R. Don, Archana Santhanam

    Affiliation(s) Name:

    Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha Institute of Technical and Medical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: P. K. Uma, Department of Oral and Maxillofacial Pathology, Saveetha Dental College, Saveetha Institute of Technical and Medical Sciences, Saveetha University, 162, Poonamallee High Road, Chennai, Tamil Nadu, India

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    Author:

    P. K. Uma*, Pratibha Ramani, Herald. J. Sherlin, S. Gheena, Gifrina Jayaraj, K. R. Don, Archana Santhanam

    Title:Shelf life of a refrigerated FNAC(Fine needle aspiration cytology) specimen
    Journal:Drug Invention Today
    Vol(issue):12 (May)
    Year:2019
    Page No: (880-882)
  • Experimental Methods Keywords

    Methodology:specimen analysis
    Research Materials:refrigerated FNAC

Keywords

Shelf life Refrigeration FNAC

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