Journal: Drug Invention Today

Article Id: JPRS-P col-00003519
Title: Alizarin red S – A procedure for staining bones
Category: Pharmacology
Section: Research Article
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    Aim: This study is to know about the skeletal framework of fish by staining the bones using Alizarin red S. This stain is widely used to evaluate the staining bones in cell culture, for dyeing textile fabrics. It became the first natural dye to be made synthetically. Alizarin red is the main constituents for the manufacture of the madder lake pigment. Materials and Methods: The chemicals required for the procedure are Formaldehyde solution, 50% potassium hydroxide (KOH), 1% Alizarin red S, Glycerol and Distilled water. The fish were killed and dipped in water with neutral formalin. Then, it was washed to remove all sludge. The scales were removed and immersed in solution containing 1% of Alizarin red S and 50% of KOH and left undisturbed. Results: This result in the staining of bones in pinkish-violet color which helps to know about the skeletal framework. Conclusion: This in vitro study helps to know about the skeletal framework of fish through the stain Alizarin red S. Thus, the staining of bones helps in disease detection and deposition of calcium.

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    Author(s) Name:

    S. Kameswari, S. Sangeetha, Karthik Ganesh Mohanraj*

    Affiliation(s) Name:

    Department of Anatomy, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: Karthik Ganesh Mohanraj, Department of Anatomy, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

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    Author:

    S. Kameswari, S. Sangeetha, Karthik Ganesh Mohanraj*

    Title:Alizarin red S – A procedure for staining bones
    Journal:Drug Invention Today
    Vol(issue):12 (May)
    Year:2019
    Page No: (890-892)
  • Experimental Methods Keywords

    Methodology:Disease detection, Formalin fixed tissues, Skeletal framework, Staining
    Research Materials:Alizarin red S

Keywords

Disease detection Formalin fixed tissues Skeletal framework Staining

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