Journal: Drug Invention Today

Article Id: JPRS-P col-00003522
Title: In vitro α-amylase and α-glucosidase inhibitory activity of isolated fraction 2 from Plectranthus amboinicus
Category: Pharmacology
Section: Research Article
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    Background: α-amylase and α-glycosidase are enzymes that play a vital in digesting carbohydrate into glucose which, in turn, prevents the rise of postprandial blood sugar in type 2 diabetes. Objective: The objective of the present study was to investigate the in vitro α-amylase and α-glucosidase inhibitory activities of isolated fraction 1 from the leaves of Plectranthus amboinicus. Materials and Methods: Methanolic extract of P. amboinicus leaves was subjected to thin-layer and column chromatography. Fractions were eluted with hexane and ethyl acetate in the ratio of 8:2. The isolated fraction 2 was subjected to α-amylase and α-glucosidase inhibition assays. Results: Different concentrations (100, 200, 300, 400, and 500 µg/ml) of isolated fraction 2 and acarbose were subjected individually to α-amylase and α-glucosidase inhibitory assay and their absorbance was measured at 540 and 405 nm using a colorimeter. Isolated fraction 2 showed appreciable α-amylase and α-glucosidase inhibitory potential in a dose-dependent manner. The maximum percentage of inhibition was observed (78.15 ± 1.46) in alpha-amylase and 70.29 ± 0.66 in alpha-glucosidase enzymes at a concentration of 500 µg/ml of isolated fraction 2. The standard drug acarbose was shown maximum percentage of inhibition in alpha-amylase 81.78 ± 0.48% and 79.88 ± 0.33 in alpha-glucosidase at a concentration of 500 μg/ml. The results produced by isolated fraction 2 were comparable to that of acarbose – an antidiabetic drug. Conclusion: These results indicated that isolated fraction 2 exerted potent inhibitory effects against α-amylase and α-glucosidase which can be considered as a potential candidate for the management of type 2 diabetes mellitus. Further, the structure of the compound will be determined later based on the spectral data.

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    Author(s) Name:

    R. Kushali, V. Vishnu Priya, R. Gayathri, R. Sundaram*

    Affiliation(s) Name:

    Department of Biochemistry, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India

    *Corresponding author: R. Sundaram, Department of Biochemistry, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Velappanchavadi, Chennai - 600 077, Tamil Nadu, India.

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    Author:

    R. Kushali, V. Vishnu Priya, R. Gayathri, R. Sundaram*

    Title:In vitro α-amylase and α-glucosidase inhibitory activity of isolated fraction 2 from Plectranthus amboinicus
    Journal:Drug Invention Today
    Vol(issue):12 (May)
    Year:2019
    Page No: (900-902)
  • Experimental Methods Keywords

    Methodology:Extraction and Isolation,In vitro α-amylase Inhibitory Assay, Alpha-glucosidase Inhibitory Assay
    Research Materials:Plectranthus amboinicus

Keywords

α-amylase α-glucosidase Fractions Plectranthus amboinicus Type 2 diabetes

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