Journal: Drug Invention Today

Article Id: JPRS-BRPT-00003536
Title: Production and properties of biosurfactant from the local isolation of Candida spp.
Category: Biotechnology & Related Pharmaceutical Technology
Section: Research Article
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    Background: This study aimed to produce biosurfactants from a local isolate of Candida yeast and evaluate the effects of carbon and nitrogen sources. The optimum biosurfactant production conditions (including pH, temperature, fermentation time, shaker-incubator speed, and inoculum volume) were also investigated. The biosurfactants were produced from two yeast isolates, namely, Candida albicans and Candida lipolytica. Material and Methods :The ability of isolates to produce biosurfactant was based on the estimation of emulsification activity, emulsification index, surface tension, oil displacement, and critical micelle concentration (CMC). Results: Results showed that the biosurfactant yield was 9 g/l, the surface tension decreased from 71 to 28 mN/m, and the CMC concentration was 1.4%. Emulsification activity depended on the determination of production capability. The quantitative and qualitative screening outputs of isolation demonstrated that the maximum activity on the wavelength of 540 nm using sunflower oil was 1.65 and that the best effect of environmental and nutritional factors on biosurfactant production was recorded by C. lipolytica. The model condition of production was investigated by evaluating the emulsification activity for the cell-free culture medium, which contained 1% glucose as a carbon source. Furthermore, its recorded activity was 2.251. A total of 2% urea was used as a nitrogen source with an activity of 2.141 and pH 4 in (2.185) activity and temperature 30°C with 2.285 as activity and 2.241 as activity for a period 72 h in shaking incubator under 180 rpm with activity (2.017) and inoculum volume 1% with activity (2.301) effectiveness was measured at 540 nm. Conclusions: This study concludes that the C. lipolytica isolate has a high capacity for biosurfactant production. The modification of growth medium reflects a positive effect on biosurfactant production, especially when monosaccharide and urea are used as carbon and nitrogen sources, respectively.

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    Author(s) Name:

    Wael A. S. Alwaely1 *, Amal K. Ghadban1 , Inaam M. N. Alrubayae2

    Affiliation(s) Name:

    1 Department of Food Science and Biotechnology, College of Agriculture, Basra University, Basra, Iraq,

    2 Department of Biology, College of Science, Basra University, Basra, Iraq

    *Corresponding author: Wael A. S. Alwaely, Department of Food Science and Biotechnology, College of Agriculture, Basra University, Basra, Iraq.

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    Author:

    Wael A. S. Alwaely1 *, Amal K. Ghadban1 , Inaam M. N. Alrubayae2

    Title:Production and properties of biosurfactant from the local isolation of Candida spp.
    Journal:Drug Invention Today
    Vol(issue):12 (May)
    Year:2019
    Page No: (948-953)
  • Experimental Methods Keywords

    Methodology:emulsification activity, emulsification index, surface tension, oil displacement, and critical micelle concentration (CMC)
    Research Materials:Yeasts, Candida spp

Keywords

Biosurfactants Candida Emulsifier Yeasts

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