Journal: Drug Invention Today

Article Id: JPRS-MSNS-00003537
Title: Antibacterial activity of metabolites isolated from local soil Actinomycetes spp.
Category: Microbiological Study (on Natural/ Synthetic drug)
Section: Research Article
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    Background: Microbial diseases have increased in recent years and have become a threat to public health. The most threatening threat to the world is the resistance of microbes to drugs. The study aims to isolate some Actinomycetes that produce antimicrobial metabolites from different soil. Materials and Methods: A total of 150 samples of different soils were randomly collected from different parts of Hilla city and studied soils properties (pH, temperature, elemental carbon, organic carbon, and relative humidity). Actinomycetes were isolated using serial dilution and plated on the International Streptomyces Project type-2 media medium. A total of 33 Actinomycetes strains were isolated and studied their morphology and cross streaked against various human microbial pathogens. Results: Only 13 isolates showed high antibacterial against Gram-positive and Gram-negative pathogenic bacteria through primary screening. Secondary metabolic products were extracted from extracellular and intracellular and tested for its activity against pathogenic bacteria using the well diffusion method. The diameters of the inhibition zone were measured for extracellular and intracellular extracts. The diameters of the inhibition zone ranged from 13 to 33 against Gram-positive bacteria and 6.3–39.2 mm against Gram-negative bacteria for extracellular extracts and ranged from 18 to 30.5 mm against Gram-positive bacteria using intracellular extracts and from 9.5 to 32.3 mm against Gram-negative bacteria. Conclusion: The current study concludes the antibacterial activity of Actinomycetes metabolites with different spectrum and inhibition zone like those resulted from carbapenems.

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    Author(s) Name:

    Yazi Abdullah Jassaim, Eman Mohammed Jarallah*

    Affiliation(s) Name:

    Department of Biology, College of Science, University of Babylon, Hillah, Iraq

    *Corresponding Author: Eman Mohammed Jarallah, Department of Biology, College of Science, University of Babylon, Hillah, Iraq

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    Author:

    Yazi Abdullah Jassaim, Eman Mohammed Jarallah*

    Title:Antibacterial activity of metabolites isolated from local soil Actinomycetes spp.
    Journal:Drug Invention Today
    Vol(issue):12 (May)
    Year:2019
    Page No: (954-962)
  • Experimental Methods Keywords

    Methodology:Antibacterial activity
    Research Materials: Actinomycetes

Keywords

Actinomycetes Antibacterial Metabolites Soli bacteria

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