Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-0000375
Title: Analysis of Amino Acids By Paper Chromatography
Category: Analytical Chemistry
Section: Research Article
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  • Abstract

    Paper chromatography method for separation and identification of amino acids in a mixture is described. The method is based on the use of ninhydrin as a locating reagent. The procedure has been successfully applied to the study of establishing a relationship between the zone area of the separated spots and the concentration of the amino acid (s) for quantitation of the solute. A suitable solvent system n-butanol-acetic acid-water (65:15:25) has been evolved which is found to give better resolution of the components during development of chromatograms. The amino acids in the concentration range varying from 10–4 to 10–1 mol / liter has been studied and found to give satisfactory results.

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  • About the authors and Affiliations

    Author(s) Name:

    Sofia Ahmed1, Muhammad Ali Sheraz 1* , Muhammad Aminuddin1, Iqbal Ahmad1, Karamat Mahmood2 and Kefi Iqbal3

    Affiliation(s) Name:

    1Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi–74600, Pakistan
    2 Department of Chemistry, The Islamia University, Bahawalpur, Pakistan.
    3 Baqai Dental College, Baqai Medical University, Karachi, Pakistan.

    *Corresponding author.
    Muhammad Ali Sheraz
    Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza,
    Super Highway, Gadap Road, Karachi–74600, Pakistan


    Received on: 20-05-2010; Revised on: 16-06-2010; Accepted on:15-07-2010

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    Author:

    Sofia Ahmed1, Muhammad Ali Sheraz 1* , Muhammad Aminuddin1, Iqbal Ahmad1, Karamat Mahmood2 and Kefi Iqbal3

    Title:Analysis of Amino Acids By Paper Chromatography
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):1 (August)
    Year:2010
    Page No: (174-176)
  • Experimental Methods Keywords

    Methodology:Paper chromatography,
    Research Materials:amino acids,

Keywords

Paper chromatography amino acids ninhydrin qualitative and quantitative analysis.

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