Journal: Drug Invention Today

Article Id: JPRS-P col-00003804
Title: Gene-dependent cariogenicity detection in Streptococcus mutans from human oral cavity
Category: Pharmaceutics
Section: Research Article
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    Background: Dental caries is the most prevalent infectious disease in mankind and is considered a chronic disease that is associated with multiple factors, including host factors (tooth surface, saliva acquired pellicle), diet, and dental plaque (bacteria). Saliva is a complex mixture of several components. Streptococcus mutans has been implicated as cariogenic bacteria in dental caries because they can produce high levels of dental caries-causing lactic acid and extracellular polysaccharide. Materials and Methods: The examined children were required to refrain from any oral hygiene procedure and food intake during the 12 h before sampling. 5 mL of non-stimulated saliva was collected in the morning in a 15 mL sterile plastic tube, which was maintained at 4°C until brought to saliva was transferred to 1.5 mL centrifuge tubes for further analysis. Gene- dependent cariogenicity detection in S. mutans from human oral cavity. Results: The isolating of total DNA samples from 15 patients was followed by the analysis of DNA integrity of saliva. The electrophoretic separation showed the presence of DNA bands larger than 10,000 bp, with integrity of the isolated DNA and minimal degradation. The standard curve was designed based on the dilution of DNA isolated from S. mutans UA159. The melting curve for the amplification of DNA of S. mutans UA159 shows a single amplification pick, to the same temperature for all the dilutions. Conclusion: This study concludes that the specific gene is present in the sequence obtained which is responsible for gene-dependent dental caries in S. mutans present in the oral cavity. This investigation revealed that the SYBR green-based real-time PCR was accurate and useful for the absolute and relative quantification of cariogenic bacteria from oral specimens in which the Gtfh gene was responsible for dental caries is present in S. mutans. The qPCR is potentially useful for quantifying viable cariogenic pathogens in oral specimens and is applicable to oral biofilm experiments. This assay will help to elucidate the relationship between the number of viable cells in oral specimens and the oral status.

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    Author(s) Name:

    J. Prerna Jain1 , J. Selvaraj 1 *, V. Vishnupriya1 , R. Ponnulakshmi 2 , R. Gayathri 1 , K. Madhan1 , B. Shyamala devi 1

    Affiliation(s) Name:

    1 Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India,

    2 Department of Central Research Laboratory, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu, India

    *Corresponding author: Dr. J. Selvaraj, Assistant Professor in Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences,Saveetha University, Chennai - 600 077, Tamil Nadu, India.

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    Author:

    J. Prerna Jain1 , J. Selvaraj 1 *, V. Vishnupriya1 , R. Ponnulakshmi 2 , R. Gayathri 1 , K. Madhan1 , B. Shyamala devi 1

    Title:Gene-dependent cariogenicity detection in Streptococcus mutans from human oral cavity
    Journal:Drug Invention Today
    Vol(issue):12 (July)
    Year:2019
    Page No: (1532-1536)
  • Experimental Methods Keywords

    Methodology:Gene-dependent cariogenicity detection
    Research Materials:human oral cavity

Keywords

Cariogenicity detection Gtfh Human oral cavity Streptococcus mutans

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