Journal: Drug Invention Today

Article Id: JPRS-P col-00003886
Title: Genome subtraction to identify the novel therapeutic targets in Mycobacterium tuberculosis
Category: Pharmaceutics
Section: Research Article
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    Objective: Tuberculosis is a deadly infection caused by Mycobacterium tuberculosis. The disease still puts a strain to HIV in causing high mortality rates. Many pathogens have developed resistance to antibiotics; therefore, in silico approach was done to find the new drug targets with less toxicity. In this study, the proteome of M. tuberculosis was sorted out for paralogs and homologous sequence and further analyzed to identify essential genes using database of essential genes. Materials and Methods: The metabolic pathway analysis was performed by Kyoto Encyclopedia of Genes and Genomes (KEGG) Automatic Annotation Server (KAAS). The comparative examination of the metabolic pathways of the host and pathogen performed by KEGG pathway. The unique protein obtained was subjected to Basic Local Alignment Search Tool against Homo sapiens to select the non-homologous protein, in that 6 proteins with no significant similarity were found. The subcellular localization of the proteins was identified using proteome analyst specialized subcellular localization server v2.5 (PA-SUB). Results: The outcomes of comparative examination of the metabolic pathways of the host and pathogen performed by KEGG pathway database reveal 154 essential proteins involved in metabolic pathway in that 17 proteins were unique to the metabolic pathway of M. tuberculosis. Among the six proteins derived, crystallographic structure was made to protein acyl desaturase and molecular off-target analysis was carried out for that unique protein and commercial drug (ethionamide) involved in metabolic pathway. It was found that side effects caused by inhibitors targeting the drug targets possessed high side effects compared to new target proposed (acyl desaturase) since acyl desaturase has no similarities with Homo sapiens and vital function plays by protein in M. tuberculosis suggested as possible therapeutic targets.

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    Author(s) Name:

    Sujatha Saravanan, Gunasekaran Shylaja*

    Affiliation(s) Name:

    Department of Biotechnology, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India

    *Corresponding author: Gunasekaran Shylaja, Department of Biotechnology, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India

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    Author:

    Sujatha Saravanan, Gunasekaran Shylaja*

    Title:Genome subtraction to identify the novel therapeutic targets in Mycobacterium tuberculosis
    Journal:Drug Invention Today
    Vol(issue):12 (August )
    Year:2019
    Page No: (1620-1624)
  • Experimental Methods Keywords

    Methodology:Drug targets, Subtractive genomics
    Research Materials:Mycobacterium tuberculosis

Keywords

Drug targets Mycobacterium tuberculosis Subtractive genomics

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