Journal: Inter. J. of Chemical and Analytical Science

Article Id: JPRS-AC-0000394
Title: UV, HPLC Method development and quantification of eugenol isolated by preparative paper chromatography from Alcoholic extracts of different species of Ocimum
Category: Analytical Chemistry
Section: Research Article
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    Around 2 ml of 99% eugenol was taken in a clean and dry test tube. The standard eugenol was spotted on a watt man filter paper having dimension 4 X 20 cm. 95% of methanol was mixed with 5% of chloroform by ultra sonication and taken around 100 ml in a chromatographic chamber. and its (Rf) value was calculated. 5mg of each extract was drawn and dissolved in 5 ml of solvent (Meoh:Chloroform 95:5) then 0.1 ml from the above solution was applied to the paper in the form of band and all the procedure was maintained as like as standard. After complete development the paper was removed out and allowed to dry. Measure the Rf value as compared with the standard eugenol. Then the portion which resemble with the standard Rf value was cut out and dissolved in 5ml of the above solvent. The solution then taken for UV analysis. By trial and error, the method having 70 parts of methanol, 20 parts of water and 10 parts of Acetonitrile was fit strong for eugenol in an Isocratic system having column C-18 and this method was developed for both standard eugenol and eugenol present in alcoholic extracts of different species of Ocimum, and subjected for HPLC analysis.

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    Author(s) Name:

    Choudhury Golak Bihari*, Behera Manaswini, Tripathy Sujit Kumar, Jena Prabhat Kumar and Mishra Sruti Ranjan

    Affiliation(s) Name:

    Department of Pharmaceutical Analysis And Quality Assurance, Faculty of Pharmacy, Jeypore College of Pharmacy, Rondapalli, Jeypore - 764002, Koraput, Odisha, India.

    *Corresponding author.
    Golak Bihari Choudhury,
    Jeypore College of Pharmacy,
    Rondapalli, Jeypore-764002, Koraput, Odisha, India,


    Received on: 20-06-2010; Revised on: 16-07-2010; Accepted on:15-09-2010

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    Author:

    Choudhury Golak Bihari*, Behera Manaswini, Tripathy Sujit Kumar, Jena Prabhat Kumar and Mishra Sruti Ranjan

    Title:UV, HPLC Method development and quantification of eugenol isolated by preparative paper chromatography from Alcoholic extracts of different species of Ocimum
    Journal:Inter. J. of Chemical and Analytical Science
    Vol(issue):2 (January)
    Year:2011
    Page No: (3-6)
  • Experimental Methods Keywords

    Methodology:UV, HPLC method development; Quantification against standard;
    Research Materials:eugenol

Keywords

Isolation of eugenol by preparative paper chromatography; UV HPLC method development; Quantification against standard; Ocimum

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