Journal: Drug Invention Today

Article Id: JPRS-P col-00004198
Title: The effect of all-trans-retinoic acid against expression Bcl2 and Bax of Choriocarcinoma BeWo (ATCC CCL-98) cell line culture
Category: Pharmaceutics
Section: Research Article
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    Aim: Retinoic acid, which is the active ingredient of retinol and vitamin A, has a stimulating activity termination trigger the cell cycle and apoptosis. The mechanism of apoptosis is important role in cancer treatment. This study aims to determine the effect of all-trans-retinoic acid (ATRA) on the expression of Bcl-2 and Bax in BeWo choriocarcinoma cell line (ATCC CCL-98). Materials and Methods: This study used BeWo choriocarcinoma cell line (ATCC CCL-98) cultures which derived from choriocarcinoma and was exposed to ATRA. This study is divided into six experimental treatment groups ATRA with various dose of 50 µg/ml, 100 µg/ml, 200 µg/ml, 400 µg/ml, and 800 µg/ml. Results: The results obtained that there was a significant deterioration and increasing of the Bcl-2 expression and Bax expression due to ATRA administration treatment. The Bcl-2 expression was significantly decreased from the dose of 200 µg/ml (P = 0.020), 400 µg/ml (P = 0.013), and 800 µg/ml (P = 0.020), with decreased expression happened the lowest dose group of 400 µg/ml. Increased expression of Bax was significantly obtained by ATRA-exposed groups at 200 µg/ml (P = 0.005), 400 µg/ml (P = 0.000), and 800 µg/ml (P = 0.000), with highest expression of Bax at a dose of 400 µg/ml. Conclusion: This indicates that administration of ATRA decreased the expression of Bcl-2, the lowest is in the dose of 200–800 µg/ml, and increased the expression of Bax in BeWo choriocarcinoma cell line, the highest is in 400 µg/ml.

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    Author(s) Name:

    Tatit Nurseta, Rahajeng Rahajeng, Mulyo Hadi Wibowo*

    Affiliation(s) Name:

    Department of Obstetrics and Gynecology, Division of Oncology Gynaecology, Faculty of Medicine, Brawijaya University, Dr. Saiful Anwar Malang General Hospital, Jalan Jaksa Agung Suprapto No. 2, Malang 65112, East Java, Indonesia

    *Corresponding author: Mulyo Hadi Wibowo, Department of Obstetrics and Gynecology, Division of Oncology Gynaecology, Faculty of Medicine, Brawijaya University, Dr. Saiful Anwar Malang General Hospital, Jalan Jaksa Agung Suprapto No. 2, Malang 65112, East Java, Indonesia

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    Author:

    Tatit Nurseta, Rahajeng Rahajeng, Mulyo Hadi Wibowo*

    Title:The effect of all-trans-retinoic acid against expression Bcl2 and Bax of Choriocarcinoma BeWo (ATCC CCL-98) cell line culture
    Journal:Drug Invention Today
    Vol(issue):12 (October)
    Year:2019
    Page No: (2166-2171)
  • Experimental Methods Keywords

    Methodology:ATRA, Bax, Bcl-2, Cell expression, Cell line choriocarcinoma
    Research Materials:retinoic acid

Keywords

ATRA Bax Bcl-2 Cell expression Cell line choriocarcinoma

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