Journal: Journal of Pharmacy Research

Article Id: JPRS-PB-0000421
Title: In silico enzyme function prediction in hypothetical proteins of Mycobacterium bovis AF2122/97
Category: Pharmaceutical Biotechnology
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    Background: Mycobacterium bovis linked to cause bovine tuberculosis has been investigated and functionally annotated for several hypothetical proteins’ ability of enzyme activity as per the conserved domain present. Methods: As M. bovis genome codes in total 25% hypothetical proteins, we used CDD BLAST, INTERPRSCAN, PFAM and CATH domain search programs to find the enzyme functionality in these proteins. Results and discussion: Programs reported 347 hypothetical proteins posses enzymatic activity as per the enzyme domain conserved in the sequences at variable confidence levels. Conclusion: These proteins could be the markers, to investigate about their fate in the metabolism of M. bovis.

    Cite this article as: P S Kewate, R C Urade, D G Gore, M A Soni and A P Kopulwar,In silico enzyme function prediction in hypothetical proteins of Mycobacterium bovis AF2122/97,Journal of Pharmacy Research 2015,9(3),182-189.

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    Author(s) Name:

    P S Kewate1, R C Urade1, D G Gore2*, M A Soni1 and A P Kopulwar1

    Affiliation(s) Name:

    1Priyadarshini Institute of Engineering and Technology (Department of Biotechnology),
    Mouza Shivangaon, Behind CRPF Campus, Hingna Road, Nagpur 440019.
    2Sai Bioinfosys Institute of Bioinformatics Research, Plot No. 271 Raghuji Nagar, Nagpur (Maharashtra), India.440009

    *Corresponding author.
    D G Gore
    Sai Bioinfosys Institute of Bioinformatics Research,
    Plot No. 271 Raghuji Nagar,
    Nagpur (Maharashtra), India.440009


    Received on:28-01-2015; Revised on: 19-02-2015; Accepted on:22-03-2015

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    Author:

    P S Kewate1, R C Urade1, D G Gore2*, M A Soni1 and A P Kopulwar1

    Title:In silico enzyme function prediction in hypothetical proteins of Mycobacterium bovis AF2122/97
    Journal:Journal of Pharmacy Research
    Vol(issue):9 (March)
    Year:2015
    Page No: (182-189)
  • Experimental Methods Keywords

    Methodology:CDD BLAST, INTERPRSCAN, PFAM and CATH domain search programs
    Research Materials:Enzyme

Keywords

Bioinformatics Hypothetical proteins Enzyme conserved domain.

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