Journal: Drug Invention Today

Article Id: JPRS-Pcol-00004447
Title: Anti-inflammatory effect of Lactobacillus casei on human epithelial cell responses to oral pathogens
Category: Pharmacology
Section: Research Article
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    Objective: The objectives of this study were to analyze the anti-inflammatory effects of the Lactobacillus casei strain Shirota on epithelial cell expression of interleukin (IL)-8 and human beta-defensin 2 (hBD-2) in the presence of pathogenic bacteria in vitro. Methods: HaCaT epithelial-cells (1 × 105 cell/mL) were exposed to heat-killed Streptococcus mutans or Porphyromonas gingivalis (1 × 107 colony-forming units/mL) and then challenged with the probiotic L. casei train Shirota for 3, 6, and 24 h. The mRNA transcription levels of IL-8 and hBD-2 were analyzed by reverse transcription-polymerase chain reaction. In addition, cell viability was analyzed using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. Data statistically evaluated by one-way analysis of variance test. Results: L. casei does not effect the HaCaT epithelial cells viability (101.3%). IL-8 and hBD-2 transcription on HaCaT cells was increased after exposed to the heat-killed bacteria. The addition of L. casei reduced the transcription levels of IL-8; in contract, probiotics challenges increased the transcriptional expression of h-BD2 mRNA in the HaCaT cells (P < 0.05). IL-8 expression significantly reduced (P < 0.05) after the L. casei challenge. Conclusion: L. casei conveyed a beneficial immune modulation, reducing IL-8 levels, and elevating production of hBD-2. The epithelial cells showed no cytotoxic effects from L. casei. Further studies are needed to investigate the molecular processes related to this probiotic’s anti-inflammatory properties.

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    Author(s) Name:

    Armelia Sari Widyarman1 *, Endang Winiati Bachtiar2 , Boy Muchlis Bachtiar2

    Affiliation(s) Name:

    1 Department of Microbiology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia,

    2 Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia

    *Corresponding author: Armelia Sari Widyarman, Department of Microbiology, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia.

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    Author:

    Armelia Sari Widyarman1 *, Endang Winiati Bachtiar2 , Boy Muchlis Bachtiar2

    Title:Anti-inflammatory effect of Lactobacillus casei on human epithelial cell responses to oral pathogens
    Journal:Drug Invention Today
    Vol(issue):12 (November )
    Year:2019
    Page No: (2605-2610)
  • Experimental Methods Keywords

    Methodology:Anti-inflammatory effect
    Research Materials:Lactobacillus casei

Keywords

Cytokine Gene expression Human beta-defensins-2 Interleukin-8 Lactobacillus casei

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