Journal: Journal of Pharmacy Research
Title: Antibacterial activity and TLC Bioautography of Ocimum basilicum L. against pathogenic bacteria
Category: Pharmaceutical Biotechnology
Section: Research Article
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Abstract
The study was designed to examine the in vitro antibacterial activity of Ocimum basilicum Linn. Aerial parts of Ocimum basilicum were extarcted with organic solvents (ethanol, methanol, ethyl acetate and hexane) and then evaluated for in vitro activity by using agar well diffusion method against Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa, Escherischia coli, and Salmonella typhi. The methanol extract showed prominent effect on the tested microorganisms. However, Salmonella typhi was found to be most resistant against the extracts. The MIC and MBC of methanol extract against Staphylococcus aureus, Bacillus subtilis and Bacillus cereus were found to be 0.625 and 1.250 mg/mL ,respectively whereas it was 1.250 and 2.500 mg/mL against Escherischia coli. The TLC analysis of methanol extract showed the appearance of seven spots. The compounds eluted at Rf 0.14, Rf 0.25 and Rf 0.35, exhibited strong antibacterial activity against Staphylococcus aureus.The results provide evidence that Ocimum basilicum might be prominent sources of preservatives in food products.
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About the authors and Affiliations
Author(s) Name: Prakash Chandra Gupta1* , Ritu Batra2, Abhishek Chauhan3, Pankaj Goyal3 and Purshotam Kaushik3
Affiliation(s) Name: 1*Microbiology Laboratory,Homoeopathic Pharmacopeia Laboratory,Ghaziabad, 201002.
2Department of Biotechnology,Career Institute of Technology and Management, Faridabad, India
3Department of Botany and Microbiology,Gurukul Kangri University Haridwar -249404, U.K., India
For correspondence:Prakash Chandra Gupta,Microbiology Laboratory,Homoeopathic Pharmacopeia Laboratory,Ghaziabad, 201002.
Received on:21-10-2008; Accepted on :06-01-2009-
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Author: Prakash Chandra Gupta1* , Ritu Batra2, Abhishek Chauhan3, Pankaj Goyal3 and Purshotam Kaushik3
Title: Antibacterial activity and TLC Bioautography of Ocimum basilicum L. against pathogenic bacteria Journal: Journal of Pharmacy Research Vol(issue): 2 (March) Year: 2009 Page No: (407-409) -
Experimental Methods Keywords
Methodology: Antibacterial activity,Minimum inhibitory concentration, TLC Bioautography. Research Materials: Ocimum basilicum
Keywords
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