Journal: Drug Invention Today

Article Id: JPRS-Pcol-00004554
Title: Effect of Nepenthes ampullaria Jack extract on the cell growth, cell membrane integrity, and morphology of mycobacterial cells
Category: Pharmacology
Section: Research Article
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    Objective: The study is aimed to evaluate the antimycobacterial properties of Nepenthes ampullaria extracts and to investigate its effect on cellular growth, cell wall integrity, and morphology of mycobacterial cell. Materials and Methods: N. ampullaria root was obtained from Johor National Park, Endau Rompin, and was extracted with methanol, water, ethyl acetate and n-hexane. The antimycobacterial activity of the extracts was tested using microdilution assay. The time-kill assay, leakage of compound absorbing at 280 nm, and field emission scanning electron microscopy were carried out to investigate the effect of the extract on cellular growth, membrane integrity, and morphology. The bioactive compounds present in the crude extract were investigated using gas chromatography–mass spectrometry (GC–MS). Results: The hexane extract of N. ampullaria demonstrated the best antimycobacterial activity against Mycobacterium smegmatis (minimum inhibitory concentration [MIC] and minimum bactericidal concentration values of 0.39 and 1.56 mg/mL, respectively). At 3-fold of MIC, hexane extract of N. ampullaria killed the entire bacterial cell within 8 h of exposure by causing the cell lysis. The GC–MS analysis revealed the presence of phytoconstituents that might contribute to the antimycobacterial effect. Conclusion: The study demonstrated the antimycobacterial properties of N. ampullaria and further studies could lead to the development of new antituberculosis agents.

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    Author(s) Name:

    Shuaibu Babaji Sanusi 1,2 , Mohd Fadzelly Abu Bakar1 *, Maryati Mohamed1 , Siti Fatimah Sabran1 , Azizul Isha3

    Affiliation(s) Name:

    1 Department of Technology and Natural Resources, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, Pagoh Educational Hub, 84600 Pagoh, Johor, Malaysia,

    2 Department of Microbiology, Faculty of Science, Kaduna State University, Tafawa Balewa Way, PMB 2339, Kaduna, Nigeria,

    3 Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

    *Corresponding author: Mohd Fadzelly Abu Bakar, Department of Technology and Natural Resources, Faculty of Applied Sciences and Technology, Universiti Tun Hussein Onn Malaysia, Pagoh Educational Hub, 84600 Pagoh, Johor, Malaysia.

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    Author:

    Shuaibu Babaji Sanusi 1,2 , Mohd Fadzelly Abu Bakar1 *, Maryati Mohamed1 , Siti Fatimah Sabran1 , Azizul Isha3

    Title:Effect of Nepenthes ampullaria Jack extract on the cell growth, cell membrane integrity, and morphology of mycobacterial cells
    Journal:Drug Invention Today
    Vol(issue):11 (December [ Special Issue 2])
    Year:2019
    Page No: (209-215)
  • Experimental Methods Keywords

    Methodology:Antimycobacterial activity, Membrane integrity
    Research Materials: Mycobacterium smegmatis, Nepenthes ampullaria

Keywords

Antimycobacterial activity Membrane integrity Mycobacterium smegmatis Nepenthes ampullaria

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