Journal: Drug Invention Today

Article Id: JPRS-Pcol-00004561
Title: Preliminary phytochemical screening and cytotoxic activity of Indian medicinal plant against human breast cancer cell line
Category: Pharmacology
Section: Research Article
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    Objectives: The objectives of the study were to evaluate the cytotoxic activity of Alstonia scholaris (Bark) against human breast cancer cell line Michigan Cancer Foundation-7 (MCF-7) using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: The bark part of A. scholaris was subjected to Soxhlet extraction using hexane, ethyl acetate, ethanol, and aqueous solvents based on their polarity. The crude extracts obtained were subjected to preliminary phytochemical analysis and tested for cytotoxicity activity against human breast cancer cell line (MCF-7) using MTT assay. Results: Phytochemical screening of different extracts, i.e., hexane, ethyl acetate, ethanol, and aqueous extracts of A. scholaris revealed the presence of alkaloids, flavonoids, terpenoids, steroids, cardiac glycosides, etc., as major components. The cytotoxic activity of four different extracts was studied using MCF 7 cell line. The ethanol extract of A. scholaris showed significant cytotoxic effect when compared to other extracts. Conclusion: The results obtained indicated that the ethanol extract of A. scholaris has excellent cytotoxic activity, and further studies will be performed to isolate the novel compounds for chemotherapeutic use.

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    Author(s) Name:

    V. Jayashree, Malarkodi Velraj*

    Affiliation(s) Name:

    Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Chennai, Tamil Nadu, India


    *Corresponding author: Dr. Malarkodi Velraj, Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels Institute of Science, Technology and Advanced Studies, Chennai, Tamil Nadu, India.

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    Author:

    V. Jayashree, Malarkodi Velraj*

    Title:Preliminary phytochemical screening and cytotoxic activity of Indian medicinal plant against human breast cancer cell line
    Journal:Drug Invention Today
    Vol(issue):11 (December [ Special Issue 2])
    Year:2019
    Page No: (246-249)
  • Experimental Methods Keywords

    Methodology:Cytotoxic activity, Phytochemical screening
    Research Materials: Alstonia scholaris

Keywords

3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide assay Alstonia scholaris Cytotoxic activity Phytochemical screening

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